苯酚降解菌CM-HZX1菌株的分离、鉴定及降解性能研究

Isolation,identification and characterization of phenol-degrading strain CM-HZX1

从污水处理厂的活性污泥中分离出一株以苯酚为唯一碳源生长的高效降解苯酚菌CM-HZX1.通过形态特征、生理生化及16S r DNA基因序列分析,初步鉴定菌株属于红球菌(Rhodococcus sp.),16S r DNA在Gene Bank的登录号为KM014567.实验结果表明,菌株CM-HZX1培养及降解苯酚的最适条件为p H=7.0,温度30℃,转速为150 r·min^(-1).该菌株能耐受4%的盐度,适应性强.0.5 g·L^(-1)苯酚在24 h时的降解率可达93.6%,1.5 g·L^(-1)苯酚在48 h时的降解率在90%以上.研究表明,该菌株在处理工业含酚废水方面具有广阔的应用前景.

A strain CM-HZX1 which can use phenol as the sole carbon source,was isolated from activated sludge of the sewage treatment plant.The strain CM-HZX1,was tentatively identified as Rhodococcus sp. based on morphological and physiological-biochemical characteristics and 16 S r DNA sequence,with the accession number of KM014567 deposited in Genbank.The results indicate that the optimal conditions for the growth of strain CM-HZX1 and its degradation of phenol are p H 7,30 ℃,150 r·min-1of shaking. It shows strong adaptability in 4% Na Cl aqueous solution. Its phenol degradation removal efficiency was up to 93.6% with in 24 hours with an initial phenol concentration of 0.5 g·L-1,and was up to 90% with in 48 hours under initial phenol concentration of 1.5 g·L-1.The strain would have a good application potential for the treatment of phenol-containing industrial wastewater.