中哈边境阿拉山口口岸输入性猫栉首蚤指名亚种线粒体COI和COII基因序列分析

COI and COII sequences analysis for an imported Siphonapterasample of Ctenocephalides felis felis at Alataw Pass,China-Kazakhstan

目的分析阿拉山口口岸地区输入性猫栉首蚤指名亚种细胞色素C氧化酶Ⅰ(COI)和Ⅱ(COII)基因特征和系统进化关系。方法从2014年1月入境集装箱死猫体表采集蚤类样本,形态学鉴定完毕后提取DNA,PCR扩增COI和COII基因并测定序列,使用Mega 6.0通过ML法构建系统发育树。结果猫栉首蚤指名亚种COI和COII基因富含A+T,碱基突变多为置换突变,无移码,缺失和插入突变;Blast显示与澳大利亚猫栉首蚤指名亚种同源性较高(99%)。结论 COI基因序列存在足够的变异能够区分亲缘关系很近的种类,为外来或新发现的蚤种的鉴别提供了分子水平的技术依据。

To analyze the molecular of cytochrome c oxidase subunit I （COI） and subunit II （COII） of mitochondrial DNA and the phylogenetic relationship of Ctenocephalides fells fells at Alataw Pass,China-Kazakhstan,blood feeding Siphonaptera were collected from cat and DNA was extracted after morphological Identification. The COI and COII gene sequence were am- plified. The phylogenetic trees was constructed by Mega 6.0 based on the nucleotide sequences of Siphonaltera published in the GenBank and the homology was analyzed. Result showed that the A＋T in the amplified COI and COII fragments of Ctenocephalides fells fells were rich. Ctenocephalides fells fells were 99 G closely related to Ctenocephalides fells fells in Australia. It＇s suggested that COI nucleotide identification could be used to classify species of Siphonaptera and provide molecular basis for identifying the foreign Siphonaptera species at Alataw Pass or other frontier ports.