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An episomal CRISPR/Cas9 system to derive vector-free gene modified mammalian cells 被引量：1

An episomal CRISPR/Cas9 system to derive vector-free gene modified mammalian cells
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摘要 Dear Editor,CRISPR and CRISPR-associated （Cas） proteins play their adaptive immunity role in degrading foreign nucleic acids in both bacteria and archaea. CRISPP,/Cas has proved efficient in modifying mammalian genomes （Cong et al., 2013; Mali et al., 2013） and various delivery methods for Cas9： gRNA complex have been established. Among different delivery means, in vitro transcribed Cas9 mRNNgRNA, Dear Editor,CRISPR and CRISPR-associated （Cas） proteins play their adaptive immunity role in degrading foreign nucleic acids in both bacteria and archaea. CRISPP,/Cas has proved efficient in modifying mammalian genomes （Cong et al., 2013; Mali et al., 2013） and various delivery methods for Cas9： gRNA complex have been established. Among different delivery means, in vitro transcribed Cas9 mRNNgRNA,
作者 Li, Linlin Gao, Fei Wu, Sen
机构地区 China Agr Univ
出处 《Protein & Cell》 SCIE CAS CSCD 2016年第9期689-691,共3页 蛋白质与细胞（英文版）
分类号 Q78 [生物学—分子生物学] TQ460.6 [化学工程—制药化工]
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参考文献10

  • 1Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA et al (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819-823.
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Protein & Cell
2016年 第9期

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