摘要
Dear Editor,CRISPR and CRISPR-associated (Cas) proteins play their adaptive immunity role in degrading foreign nucleic acids in both bacteria and archaea. CRISPP,/Cas has proved efficient in modifying mammalian genomes (Cong et al., 2013; Mali et al., 2013) and various delivery methods for Cas9: gRNA complex have been established. Among different delivery means, in vitro transcribed Cas9 mRNNgRNA,
Dear Editor,CRISPR and CRISPR-associated (Cas) proteins play their adaptive immunity role in degrading foreign nucleic acids in both bacteria and archaea. CRISPP,/Cas has proved efficient in modifying mammalian genomes (Cong et al., 2013; Mali et al., 2013) and various delivery methods for Cas9: gRNA complex have been established. Among different delivery means, in vitro transcribed Cas9 mRNNgRNA,