GSTM1和GSTP1基因多态性与卵巢癌发生风险的关系

Correlation between polymorphisms of GSTM1 and GSTP1 genes and risk of ovarian cancer

目的探讨GSTM1和GSTP1基因多态性与卵巢癌发生风险的关系。方法选择2013年1月至2015年6月在北京大学深圳医院确诊为卵巢癌的手术患者124例为病例组,选取同时期来本院体检的健康女性124例为对照组。于清晨抽取所有研究对象4m L外周静脉血,用于multiplex-PCR和PCR-RFLP检测研究对象的GSTM1和GSTP1基因的多态性,并对结果进行分析。结果病例组GSTP1基因GG型分布频率为28.2%,与健康对照组相比差异有统计学意义（χ2=5.511,P〈0.05）,且GSTP1基因GG型可能会增加患卵巢癌的风险（OR=2.259,95%CI：1.14~4.50,P〈0.05）,携带GSTP1基因GG型患者患卵巢上皮性癌的比例明显高于卵巢非上皮性癌（χ2=4.017,P〈0.05）;而GSTM1各基因型分布与健康对照组相比差异均无统计学意义（均P〉0.05）,且与卵巢癌的发生风险无关联。结论 GSTP1基因多态性与卵巢癌的发病风险有关联,其GG型可能会增加卵巢上皮性癌的发病风险;而GSTM1基因则与卵巢癌的发病风险无关。

Objective To study the relationship between polymorphism of GSTM1 and GSTP1 genes and the risk of ovarian cancer.Methods Totally 124 patients with ovarian cancer identified in Peking University Shenzhen Hospital between January 2013 and June 2015 were selected in case group. At the same period 124 healthy females for physical examination in the hospital were selected in control group.In morning 4 m L peripheral venous blood was drawn from all cases for multiplex-PCR and PCR-RFLP to detect GSTM1 and GSTP1 gene polymorphism. Results were analyzed. Results The GG genotype frequency of GSTP1 gene in the case group was 28. 2%,which was significantly different from that in the control group（ χ2= 5. 511,P〈0. 05）. And GG genotype of GSTP1 gene might increase the risk of ovarian cancer（ OR = 2. 259,95% CI： 1. 14- 4. 50,P〈0. 05）. The cases carrying GG genotype had a higher proportion of developing ovarian epithelial neoplasms than non-epithelial ovarian cancer（ χ2= 4. 017,P〈0. 05）. However,the distribution of genotypes of GSTM1 was not significantly different from that of the control group（ P〉0. 05）,and there was no association with the risk of ovarian cancer.Conclusion There is a link between GSTP1 gene polymorphism and the risk of ovarian cancer,and GG genotypes may increase the risk of ovarian epithelial neoplasms. But GSTM1 gene is not associated with the risk of ovarian cancer.