日内瓦毛霉对妊娠烯醇酮的生物转化研究

Bio-Transformation of Pregnenolone by Mucor Genevensis

目的探索日内瓦毛霉微生物对妊娠烯醇酮的生物转化问题。方法将妊娠烯醇酮经生物转化后,发生7-羟基化,得到两种产物,通过红外、核磁、质谱分析,确定其化学结构进行优化研究。结果碳源、氮源对转化率的影响很大,试验最佳的碳源、氮源确定为葡萄糖和蛋白胨,辅助氮源为酵母膏。结论通过单因素条件优化,确定碳源和氮源加量分别为3.6%和1.3%。对不同浓度无机盐KH2PO4对转化率的影响,确定其浓度为0.16%,初始p H为4.5时,最终得到主产物的转化率为59.2%。

Microbial transformation of pregnenolone was studied in the Mucor genevensis culture,in which 7α,11α-dihydroxypregnenolone and 7α,11 β- dihy droxypregnenolone were given from the metabolites. The biotransformation products were determined by IR,NMR and MS analysis. The results showed that the optimal condition for the biosynthesis of 7α,11α—dihydr-roxy-pregnenolone was as follows： 3.5 %dextrose,1.4% peptone,0.16% potassium dihydydrogen phosphate,initial p H 4.5. The yield of7α,11α-dihydroxypregnenolone was 59.2%.