TGF-β、FGF-2和Ezrin在金黄地鼠口腔癌模型中的表达

Expression of TGF-β,FGF-2 and Ezrin in golden hamster oral cancer model

目的研究TGF-β、FGF-2和Ezrin在7,12-二甲基苯并[A]蒽(7,12-dimethyl-benzo[A]anthracene,DMBA)诱导的金黄地鼠颊黏膜癌模型中的表达,探讨其在口腔癌癌变中的作用。方法在金黄地鼠的单侧颊囊黏膜涂抹0.5%的DMBA,获得不同病变组黏膜标本,进行免疫组织化学检测和相关性分析。结果在上皮中至重度异常增生和早期癌变组中,TGF-β的高表达率分别为75%和100%,FGF-2的高表达率为83%和72%,而Ezrin高表达率分别为79%和77%,明显高于正常黏膜组和单纯及轻度异常增生组,差异均有统计学意义(均P<0.01)。相关性分析显示,三者表达呈正相关(P<0.01)。结论 TGF-β、Ezrin、FGF-2可能共同在口腔癌癌变过程中起重要作用。

Objective To investigate the expression of TGF-β,Ezrin and FGF-2 in 7,12- dimethyl-benzo [A]anthracene（ DMBA） induced golden hamster oral cancer model and evaluate the role of TGF-β,Ezrin and FGF-2 in oral mucosal precancerosis process. Methods The unilateral cheek mucosa of the golden hamsters was treated with 0. 5% DMBA and a series of samples were obtained. The expression of TGF-β,Ezrin and FGF-2 was examined by immunohistochemistry. Meanwhile,the correlation between the expressions of these three factors was analyzed. Results In moderate to severe epithelial dysplasia and early cancer groups the high TGF-β-positive rate was 75% and 100% respectively,while high FGF-2-posotive rates was 83% and 72% and high expression rate of Ezrin was 79% and 77%. Compared with mild epithelial dysplasia group and normal mucosa group,there was a significant difference（ P 〈0. 01）. Furthermore,there was a significant correlation among the expressions of TGF-β,Ezrin and FGF-2. Conclusions TGF-β,Ezrin and FGF-2 may play an important role and have an interaction effect in the carcinogenesis of oral squamous cell carcinoma.