活性氧测定分析方法的建立以及应用

Establishment and Application of Analytical Method for Determination of Reactive Oxygen Species

对活性氧物质检测的方法有很多种,其中色谱法即活性氧物质与特定指示剂发生特异性反应后通过高效液相色谱法(HPLC)分离得以定量分析。羟基自由基和单线态氧分别有各自特定的指示剂4-氯苯甲酸和糠醇,羟基自由基和单线态氧与4-氯苯甲酸和糠醇可以发生1:1的特异性反应,然后用HPLC进行分析,进一步可以推测出羟基自由基和单线态氧的浓度。利用这个原理建立活性氧测定分析方法。含有Fe^(3+)、富里酸(FA)、NO_3^-的水溶液在光照下可以产生活性氧物质,用上述建立的HPLC分析方法测定溶液中羟基自由基和单线态氧产生以及含量。

There are many methods of reactive oxygen species detection. Among them, the chromatography is to use high performance liquid chromatography（HPLC） to realize quantitative analysis after specific reaction between reactive oxygen species and specific indicator. Hydroxyl radicals and singlet oxygen respectively have their specific indicator 4-chlorobenzoate and furfuryl alcohol, and 1：1 specific reaction between them can form, and then HPLC analysis can further infer the concentration of hydroxyl radicals and singlet oxygen. Based on the principle, the analytical method for determination of reactive oxygen species can be established. In this paper, production of reactive oxygen species in aqueous solution containing fulvic acid（FA）, Fe^（3＋）, NO_3^- under the sunlight illumination was studied. With the established HPLC analysis method, the content of hydroxyl radicals and singlet oxygen in the aqueous solution containing Fe^（3＋）, NO_3^- and FA was measured.