Effect of moxibustion at Shenshu(BL 23) on the ethology,corticosterone and glucocorticoid receptor in aging rats

艾灸肾俞穴对衰老大鼠行为学、皮质酮及糖皮质激素受体的影响（英文）

Objective：To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor（GR） in subacute aging rats.Methods：Twenty four Sprague-Dawley（SD） rats were randomly divided into a normal group,a model group and a moxibustion group,8 rats in each group.Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/（kg·bw）] for 40 d continuous;rats in the normal group were injected with saline at the same position for 40 d continuous.Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu（BL 23） at the same time of modeling;rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d.The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment.Abdominal aorta blood and thymus were collected after water maze experiment.Enzyme-linked immunosorbent assay（ELISA） was used to detect serum corticosterone level,and immunohistochemical method was used to detect the expression of thymus GR.Results：Compared with the normal group,rats in the model group showed that a significantly longer escape latency time（P〈0.01） on the third and the fourth days;number of times crossing the platform in 70 s significantly reduced（P〈0.01）;activity times in the fourth quadrant significantly decreased（P〈0.05）;serum corticosterone levels increased（P〈0.01）;thymus GR expression decreased（P〈0.05）.Compared with the model group,rats in the moxibustion group showed that the escape latency times were significantly shorter on the third,the fourth and the fifth days（P〈0.01,P〈0.05）;number of times crossing the platform in 70 s significantly increased（P〈0.05）;activity times in the fourth quadrant significantly increased（P〈0.05）;serum corticosterone levels decreased（P〈0.05）;thymus GR expression increased（P〈0.05）.Conclusion：Moxibustion could improve the learning and memory abilities of subacute aging rats,down-regulate serum corticosterone levels,and increase thymus GR content.

目的:观察艾灸对亚急性衰老大鼠学习记忆能力和皮质酮及糖皮质激素受体(glucocorticoid receptor,GR)的影响。方法:将24只(Sprague-Dawley,SD)大鼠按随机数字表分为正常组、模型组和艾灸组,每组8只。模型组和艾灸组按每日125 mg/(kg·bw)于颈背部皮下注射25%的D-半乳糖,连续40 d;正常组大鼠同部位注射等量生理盐水,连续40 d。艾灸组大鼠造模同时给予温和灸双侧肾俞,正常组和模型组只做相同抓取,不艾灸,共40 d。实验结束,采用M orris水迷宫实验观察大鼠学习记忆能力,水迷宫实验结束后取腹主动脉血和胸腺,酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法检测血清皮质酮含量,免疫组化法检测胸腺G R表达情况。结果:与正常组比较,模型组大鼠逃避潜伏期时间在第3天和第4天显著延长(P<0.01),70 s内穿越平台次数显著减少(P<0.01),在第四象限的活动时间明显减少(P<0.05);血清皮质酮含量升高(P<0.01);胸腺G R表达下降(P<0.05)。与模型组比较,艾灸组大鼠逃避潜伏期时间在第3天、第4天和第5天显著缩短(P<0.01或P<0.05),70 s内穿越平台次数显著增多(P<0.05),在第四象限的活动时间明显增多(P<0.05);血清皮质酮含量降低(P<0.05);胸腺G R表达升高(P<0.05)。结论:艾灸可改善亚急性衰老大鼠学习记忆能力,下调血清皮质酮含量,上调胸腺G R含量。