摘要
目的:探讨额尔敦-乌日勒对大脑中动脉阻塞/再灌注损伤(Middle Cerebral Artery Occlusion/Reperfusion,MCAO/R)大鼠皮质脑源性神经营养因子(Brain-Derived Neurotrophic Factor,BDNF)及神经生长因子(Nerve Growth Factor,NGF)的影响。方法:取清洁级健康SD大鼠60只,随机分为假手术组、模型组、尼莫地平片组、额尔顿-乌日勒组,采用改良Zea-Longa法,制备MCAO/R模型。实验结束后,各组大鼠麻醉、取脑,采用HE染色、SP等免疫组化方法评价脑组织病理形态学的改变情况,并且采用双抗夹心ELISA法和荧光定量RT-PCR法检测大鼠脑前额叶皮质BDNF m RNA、NGF m RNA相对表达量及其蛋白含量。结果:与模型组相比,额尔敦-乌日勒组大鼠脑前额叶皮质坏死细胞数显著减少(P<0.05),BDNF、NGF蛋白含量及m RNA表达明显增加(P<0.05)。结论:额尔敦-乌日勒可上调MCAO/R大鼠脑前额叶皮质BDNF、NGF表达,促进星形胶质细胞活化,从而保护神经元,进而起到脑保护作用,这可能是额尔敦-乌日勒治疗"白脉"病的传统功效机制之一。
The present study aimed to explore the effects of Eerdun-Wurile on brain-derived neurotrophic factor（BDNF） and nerve growth factor（NGF） expressions in the prefrontal cortex of MCAO / R injury rats.Sixty SD male rats of SPF grade were selected to establish the model of MCAO / R with Zea-Longa thread occlusion,and divided into five groups at random：the sham operation group,the model group,the nimodipine group and the Eerdun-Wurile group.After modeling,rats were anesthetized for preparing the brains.The pathomorphological changes of the brains were evaluated by immunohistochemical techniques,such as HE staining and SP.The protein and m RNA expressions of BDNF and NGF in the prefrontal cortex of rats were detected by ELISA and RT-PCR,respectively.As a result,compared with the model group,it was found that the number of necrotic cells in the prefrontal cortex were significantly reduced in the Eerdun-Wurile group（P 〈 0.05）,while the m RNA and protein expressions of BDNF and NGF were significantly increased（P 〈 0.05）.In conclusion,the BDNF and NGF expressions in the prefrontal cortex were up-regulated for stimulating the activation of astrocytes and protecting the neurons with the treatment of Eerdun-Wurile in MCAO / R injured rats,which may be the mechanism of the treatment of Eerdun-Wurile for＂ white vein＂ disease.
出处
《世界科学技术-中医药现代化》
2016年第7期1212-1218,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81560700):额尔敦-乌日勒预处理对心肌缺血再灌注损伤的作用及其复杂机制研究
负责人:麻春杰
内蒙古医科大学博士启动基金项目(2014YKDXBSJJ15):额尔敦-乌日勒中挥发性成分的定性研究
负责人:莲花
内蒙古医科大学青年创新基金项目(YKDX2014QNCX018):额尔敦-乌日勒血清药物化学的初步研究
负责人:莲花
