摘要
本文考察了罗非鱼皮胶原酶解物(TSCH)对双醛淀粉(DAS)交联明胶膜理化性质的影响,探讨了膜对人皮肤永生化角质(Ha Cat)细胞的相容性。结果发现,TSCH添加量从0增加至16%时,DAS交联明胶膜的抗拉伸强度从55.00 MPa下降至24.85 MPa,而断裂延伸率随着TSCH添加量的增加呈现先增大后减小的趋势。DAS交联明胶膜在30℃水中的溶解率为33.10%,当膜中TSCH含量超过4%后,膜的溶解率接近100%。然而,不管TSCH添加量是多少,经过240 min后膜中含有的TSCH全部释放到水中。SDS-PAGE的结果表明,在DAS交联明胶膜中添加的TSCH会阻碍明胶蛋白分子的相互交联作用。另一方面,将Ha Cat细胞培养于DAS交联明胶膜时,Ha Cat细胞活力只有11.39%,当DAS交联明胶膜中含有8%的TSCH时,Ha Cat细胞活力可以达到108.20%。以上结果表明,添加TSCH的DAS交联明胶膜在化妆品领域将具有一定的应用潜力。
The effect of tilapia skin collagen hydrolysate(TSCH) on the physicochemical properties of dialdehyde starch(DAS) cross-linked gelatin films was investigated,and the biocompatibility of these films with the human skin keratinocyte(Ha Cat) cell was also studied.When the TSCH content in the DAS-gelatin films was increased from 0 to 16%,the tensile strength(TS) of films decreased from 55.00 MPa to 24.85 MPa,while the elongation at break(EAB) showed a downward trend after the initial increase with increasing amount of added TSCH.The solubility of DAS cross-linked gelatin films in water at 30 ℃ was 33.10%,and at TSCH concentration higher than four percent,it increased to 100%.However,TSCH from films was released into water after 240 min irrespective of the amount of added TSCH.The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) analysis revealed that the addition of TSCH in the DAS cross-linked gelatin film could hinder the interaction between the protein molecules of gelatin.On the other hand,when Ha Cat cell was cultured on the surface of DAS cross-linked gelatin films,the cell activity of Ha Cat cells was 11.39%.When the DAS cross-linked gelatin film contained 8% TSCH,the Ha Cat cell activity reached up to 108.2%.Based on the above results,the DAS-gelatin films containing TSCH have potential applications in the cosmetic industry.
出处
《现代食品科技》
EI
CAS
北大核心
2016年第8期134-139,47,共7页
Modern Food Science and Technology
基金
国家自然科学基金(31271984)
福建省杰出青年科学基金项目(2014J06013)
厦门市海洋经济发展专项资金(13CZP003HJ05)
闽海渔高新([2013]006号)
关键词
胶原酶解物
罗非鱼皮
明胶膜
双醛淀粉
细胞活力
角质细胞
collagen hydrolysate
tilapia skin
gelatin film
dialdehyde starch
cell viability
keratinocytes