摘要
The insecticidal efficacy of the diatomaceous earth against granary weevil, Sitophilus granarius L. （Coleoptera： Curculionidae）, with two essential oils for the degree of repellent activity, was evaluated in an experimental model in laboratory tests. The diatomaceous earth was obtained from three Romanian sources （Patirlagele, Urloaia and Adamclisi） and was applied at four doses of 100, 300, 500 and 900 ppm （5, 15, 25 and 45 mg, respectively） on three replicates each variant. Mortality of S. granarius adults was estimated after 7, 14 and 21 d of exposure on treated grain. The insecticidal efficacy of the examined diatomaceous earth against the granary weevil was highly influenced by exposure time, dose and essential oil type. The longer exposure times increased weevil mortality in the case of low doses （100 ppm and 300 ppm）. After 60 d from the experiment initiation, the exposed grains from each variant were assessed about the progeny production. For the model proposed as bio-experiment, two essential oils in concentration of 80μL/L air and 125μL/L of Thymus vulgaris L. and Satureja hortensis L., respectively, were tested compared with untreated control in laboratory conditions. Both plant species belong to the family Lamiaceae, and the major active insecticidal compound was thymol among other providing to terpenoid class, such as eugenol and linalool. The species S. granarius chosen for experimental model was grown in the Research-Development Institute of Plant Protection （RDIPP） Bucharest bio-base. The results show that mortality induced from diatomaceous earth was at the level between 83.33% and 100% in all variants after 21 d, compared with untreated control and a standard product Silicosec.
