摘要
为获得酵母胞内产物的高效分泌,以酿酒酵母二倍体(2n)及单倍体(n)为出发菌,经紫外诱变处理,筛选获得5株可能具有温度敏感性的突变株。为进一步鉴定,以野生菌Y1为对照菌,测定其胞外核酸、蛋白质及果糖-1,6-二磷酸(FDP)的含量,发现突变菌株Ts4(2n)自溶程度最高,其核酸、蛋白质渗透率达1.709,1.483,胞外FDP浓度为38μg/mL,较Y1(2n)提高了24.1%。此外突变株Ts2*(n)、Ts3*(n)也会发生一定程度的自溶。因此,该试验共获得温度敏感性突变株3株,即Ts4{(2n)、Ts2*(n)和Ts3*(n)。
In order to obtain the efficient secretion of intracellular product in yeast, as the original strain, Saccharomyces cerevisiae (2n), (n) were mutagenized with UV treatment, five mutants strain may have temperature-sensitive were selected. To further identify, Y, was used as the control bacterium, and its content of extraeellular nucleic acids, proteins and FDP were determined, find that the high- est degree of autolysis was Ts4 (2n), it's content of nucleic acid and protein in extraeellular environment were 1.709, 1.483, the concentration of FDP in extracellular environment was 38μg/mL, higher than Y1 24.1%0. Ts* (n) and Ts* (n) also have a certain extent of temperature-sensitive. Therefore, 3 temperature-sensitive mutants were obtained in this study, which are Ts4(Zn), Ts2* (n) and Ts3*(n).
出处
《食品与机械》
CSCD
北大核心
2016年第8期9-12,16,共5页
Food and Machinery
基金
清远市科技计划项目(编号:2013A024
2014A023)
湖南省科技计划项目(编号:2013FJ4036)
关键词
酿酒酵母
紫外诱变
温度敏感性突变株
自溶
Saccharornyces cerevisiae
UV mutagenesis, temperature-sensitive mutant
autolysis