冷藏条件下带蓬鲜莲优势腐败菌鉴定及其消长规律研究

The dominant spoilage bacteria and their growth and decline law in fresh lotus seeds storedat the cold storage

通过稀释平板法分离4℃条件贮藏18d的带蓬鲜莲腐败菌,提取筛选菌株DNA后进行PCR扩增,采用细菌16SrDNA菌种鉴定法对优势腐败菌进行鉴定,并分析其4℃冷藏过程中的消长规律。结果表明,带蓬鲜莲4℃冷藏过程中的优势腐败菌主要为分散泛菌(Pantoea dispersa)、表皮葡萄球菌(Staphylococcus epidermidis)、巴氏葡萄球菌(Staphylococcus pasteuri)、成团泛菌(Pantoea agglomerans)。且4℃贮藏期间,分散泛菌、表皮葡萄球菌、巴氏葡萄球菌的变化趋势大体相同,适应期后呈增长趋势,成团泛菌变化规律不明显。

In this study, spoilage bacteria in fresh Xiang lotus seeds preserved at 4℃ temperature for 18 d were separated by dilution- plate method. Total genornic DNA was extracted from these strains and then subjected to PCR amplification. Dominant spoilage bacteria were identified using 16S rDNA sequencing for the identification and the law of their growth and declination was drawn. The results showed the four dominant spoilage bacteria in fresh Xiang lotus seeds preserved at the temperature of 4℃ were Pantoea dispersa, Stapll-ylococcus epidermidis, Staphylococcus warneri, and Pantoea ag-glomerans. At the storage temperature of 4℃, the growing trends of Pantoea dispersa, Staphylococcus epidermidis, Staphylococcus warneri of fresh lotus seeds were similar, which all needed an adap- tive phase before their increase. The amount change of Pantoea alg-glomerans was irregular.