青春期双酚A结构类似物暴露对小鼠行为的影响

Effects of Exposure to Analogues of Bisphenol A on Depressive-like Behaviors in Mice

作为重要的环境内分泌干扰物,双酚A的神经发育毒性已经引起广泛关注,其结构类似物对健康影响相关研究少有报道。为探讨双酚A结构类似物长期暴露对个体抑郁行为的影响,选取60只小鼠随机分为5组,每组12只。小鼠分别灌胃给予0、5、50 mg·kg^(-1)的双酚F或双酚S,连续给药4周。给药结束后,利用强迫游泳测试和悬尾测试评价小鼠抑郁样行为,同时监测小鼠体质量增加和自发活动情况。结果表明,与溶媒对照组比较,持续4周的双酚F和双酚S给药组小鼠的体质量变化和自发活动均无显著差异;5mg·kg^(-1)的双酚F或双酚S处理均不显著改变小鼠的抑郁行为,50 mg·kg^(-1)双酚F显著增加小鼠悬尾和强迫游泳测试中的不动时间,50mg·kg^(-1)双酚S处理显著增加小鼠悬尾测试中的不动时间。研究结果提示高浓度双酚F和双酚S在青春期的暴露可导致成年后小鼠抑郁样行为异常,应当引起重视。

The adverse effects of bisphenol A（BPA） on neurodevelopment and behaviors have been well established.However,the effects of exposure to BPA analogues,bisphenol F（BPF） and bisphenol S（BPS）,on depressive-like behaviors are poorly understood. In the present study,sixty mice were randomly divided into 5 groups and received by ga-vage 0,5 or 50 mg·kg^-1of BPF or BPS for 28 continuous days. Depressive-like behaviors were tested after BPF or BPS treatment by tail suspension test（TST） and forced swimming test（FST） mice paradigms. The bodyweight increased and locomotor activities of mice were also assessed. The results showed that BPF and BPS administration had no significant effects on both bodyweight change and locomotor activities. BPF or BPS treatment at 5 mg·kg^-1dose had no significant effect on depressive-like behaviors. BPF treatment at 50 mg·kg^-1 significantly increased the immobility time in both FST and TST. BPS treatment at 50 mg·kg^-1significant increased immobility time in TST,but just had an increased trend in FST. These findings indicated that chronic exposure to high BPF and BPS could affect the depressive-like behaviors in adult mice and should be paid more attention in the future.