摘要
为比较不同粒径麸皮的多酚含量及抗氧化活性,该实验采用不同有机溶剂分别对麸皮的游离多酚和结合多酚进行提取,并测定最佳提取条件下不同粒径麸皮的多酚含量、抗氧化活性及阿魏酸含量。结果表明:游离多酚和结合多酚提取的最佳溶剂分别为体积分数80%丙酮和乙酸乙酯,其提取液的多酚含量及对DPPH·清除能力都是最高的。不同粒径麸皮其多酚含量和黄酮含量都有显著差异,结合多酚、结合黄酮、总多酚和总黄酮的含量都随粒径减小而显著降低,而游离多酚、游离黄酮含量显著增加(P<0.05),不同粒径麸皮的抗氧化活性强弱与相应的多酚含量变化一致;麸皮中的阿魏酸主要存在于结合多酚中,且随粒径的减小其含量呈极显著降低(P<0.01)。综上可知,粒径对麸皮中的多酚及抗氧化活性有显著影响。
Several organic solvents were used to extract polyphenol in wheat bran,and antioxidant activities among different particle size of wheat bran were studied. The results showed that the best extraction solvent for free phenolic acid and bound phenolic were 80% acetone and andethyl acetate,the polyphenol content and DPPH· scavenging ability were the highest. Different particle size of wheat bran had significant influence in polyphenol and flavonoid levels. The content of compound phenolic acid,bound flavonoid,total phenolic acid and total flavonoid decreased obviously with wheat bran particle size decreased,while free phenolic acid and free flavonoid increased obviously,and the trend in antioxidant property was similar with that of polyphenol content. Ferulic acid mainly existed in bound phenolic,and its content reduced significantly with the reduction of particle size in wheat bran. In conclusion,particle size of wheat bran had remarkable influences on its phenol level and antioxidant activity.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2016年第9期160-164,共5页
Food and Fermentation Industries
基金
四川省科技厅(2014NZ0078
2015ZYZF0107)
四川省经信委(2015ZYZF0107)
西华大学人才基金(R0910507)
西华大学食品生物技术省级重点实验室开放基金
西华大学创新基金资助项目(ycjj2015022)
关键词
麸皮
多酚
抗氧化活性
阿魏酸
wheat bran
phenolic acid
antioxidant activity
ferulic acid