摘要
以秀珍菇子实体为原料,经闪式提取、Sevag法脱蛋白、不同孔径的滤膜分离,得到秀珍菇子实体多糖（PFP-m）。采用光谱分析和比色等方法对其部分结构和性质进行测定。结果表明：PFP-m为均一组分,分子质量为3.27×10~4Da;具有多糖的特征吸收峰;PMP衍生化结合HPLC法得出其单糖组成及摩尔比为甘露糖∶鼠李糖∶葡萄糖∶木糖∶阿拉伯糖=3.10∶1.00∶22.99∶6.85∶4.59。PFP-m具有多糖的一般理化性质,总糖含量为93.16%。与柱层析法相比,膜超滤法操作简单,效率高,适合秀珍菇子实体多糖PFP-m的分离纯化。
A polysaccharide（ PFP-m） was obtained from the fruit body of Pleurotus geesteranus by a sequential processing of flash extraction,deproteinization and separation through different pore ultrafiltration membrane. Its partial properties and structure were studied by spectrographic method and colorimetry. The results showed that PFP-m was a homogeneous polysaccharide. Its Mw was 3. 27 × 10~4 Da. Typical polysaccharide absorption of PFP-m was in its IR spectrum. PFP-m consists of Man,Rha,Glc,Xyl,Ara,in molar ratios of 3. 10 ∶ 1. 00 ∶ 22. 99 ∶ 6. 85 ∶ 4. 59 by PMP-HPLC. PFP-m has general physical and chemical properties of polysaccharide and its total sugar content were93. 16 %. Compared with column chromatography,ultrafiltration membrane method was easier to operate and more efficient. It was suitable for separation and purification of polysaccharide PFP-m from pleurotus geesteranus fruit body.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2016年第9期165-168,共4页
Food and Fermentation Industries
基金
安徽省高等学校省级自然科学研究重点项目(KJ2014A052)
安徽大学现代生物制造协同创新中心2015年度开放课题(BM2015004)
安徽省高校2016年优秀青年人才支持计划重点项目(gxyq ZD2016211)
安徽科技学院重点学科项目(AKZDXK2015B02)
关键词
秀珍菇
子实体
多糖
膜分离
Pleurotus geesteranus
fruit body
polysaccharide
membrane separation
