摘要
In this study, we develop the gelatin-dopamine （Gel-Dopa） nano-gels （GDNGs） and explore their potential as drug delivery vehicles. The GeI-Dopa precursor is synthesized using EDC/NHS coupling reaction, in which the catechols can coordinate with transition metal ions such as Fe^3＋. These novel GDNGs exhibit excellent cytocompatibility. The model drug, doxorubicin （Dox）, is readily conjugated into catechol of GDNGs by the coordination cross-link of Fe^3＋ ion. The morphology and size distribution of the nanogels are characterized via field emission scanning electron microscopy and particle size analyzer, respectively. The GDNGs loaded with Dox （GDNGs-Dox） is capable of efficiently penetrating ceil membrane and enter the HeLa cells. The endocytosed GDNGs-Dox release Dox molecules and subsequently kill the tumor cells.
In this study, we develop the gelatin-dopamine （Gel-Dopa） nano-gels （GDNGs） and explore their potential as drug delivery vehicles. The GeI-Dopa precursor is synthesized using EDC/NHS coupling reaction, in which the catechols can coordinate with transition metal ions such as Fe^3＋. These novel GDNGs exhibit excellent cytocompatibility. The model drug, doxorubicin （Dox）, is readily conjugated into catechol of GDNGs by the coordination cross-link of Fe^3＋ ion. The morphology and size distribution of the nanogels are characterized via field emission scanning electron microscopy and particle size analyzer, respectively. The GDNGs loaded with Dox （GDNGs-Dox） is capable of efficiently penetrating ceil membrane and enter the HeLa cells. The endocytosed GDNGs-Dox release Dox molecules and subsequently kill the tumor cells.
基金
supported by the AcRF Tier 2 ARC 1/13 of the Ministry of Education, Singapore
the National Natural Science Foundation of China (No. 51328301)
