针刺不同穴组对慢性疲劳综合征小鼠行为及抗氧化能力的影响

Effects of acupuncture of different acupiont groups on behaviors and antioxidant capacities in mice with chronic fatigue syndrome

目的比较针刺不同穴组对慢性疲劳综合征(CFS)小鼠的治疗作用。方法雄性昆明小鼠60只,适应性饲养3 d后随机分为正常组、模型组、"合谷、太冲"组、"合谷、太冲、三阴交"组、"合谷、太冲、足三里"组,每组12只。采用慢性束缚+力竭游泳复合因素建立CFS模型,共12 d。治疗7 d后观察各组小鼠行为学改变,检测血清抗氧化指标。结果 1模型组与正常组比较,悬尾不动时间延长,水平运动、垂直运动得分减少,血清丙二醛(MDA)含量增加、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)活性下降,差异有统计学意义(P<0.01)。2各针刺组与模型组比较,悬尾不动时间缩短,水平运动、垂直运动得分增加,血清MDA含量减少,SOD及GSH-PX活性升高,差异有统计学意义(P<0.05),其中"合谷、太冲、足三里"组改善最为显著(P<0.01)。3"合谷、太冲、足三里"组与其余两针刺组比较,血清MDA含量减少、SOD及GSH-PX活性升高,差异有统计学意义(P<0.05)。结论针刺各组腧穴均能对CFS小鼠起到明显的治疗作用,其中"合谷、太冲、足三里"穴组治疗效果最佳,其配伍作用更符合CFS的基本病机。

Objective To compare the therapeutic effects of acupuncture of different acupoint groups in mice with chronic fatigue syndrome（CFS）.Methods After 3 days of adaptive feeding,60 KM male mice were randomly divided into normal group, model group, ＂ Hegu（ LI4）, Taichong（ LR3） ＂ group, ＂ Hegu（ LI4）, Taichong（ LR3）, Sanyinjiao（SP6）＂ group, ＂Hegu（LI4）,Taichong（LR3）,Zusanli（ST36）＂ group.And 12 mice were in each group.CFS model was established by chronic restraint and exhaustive swimming for 12 days.After 7 days of treatment,the behavioral changes of the mice were observed,and the serum antioxidant indices were detected. Results 1 Model group was compared with the normal group,immobility time in tail suspension test was longer;scores of horizontal movement and vertical movement were less;serum malondialdehyde（MDA） content was more;serum superoxide dismutase（SOD） activity and glutathione peroxidase（GSH-PX） activity were lower;the differences were statistically significant（ P〈0.01）. 2 Each acupuncture group was compared with model group,immobility time in tail suspension test was shorter;scores of horizontal movement and vertical movement were more;serum MDA content was less;serum SOD activity and GSH-PX activity were higher;the differences were statistically significant（ P〈0.05）.The improvement of ＂LI4,LR3,ST36＂ group was the most significant（P〈0. 01）. 3 ＂ LI4, LR3, ST36 ＂ group was compared with other acupuncture groups, serum MDA content was less; serum SOD activity and GSH-PX activity were hig her;the differences were statistically sig-nificant（ P〈0. 05）. Conclusion Each acu point group has an obvious therapeutic effect in mice with CFS,in which, ＂ LI4, LR3, ST36 ＂ has a better efficacy than other acupoint groups,and the effect of the combination is more in line with the basic pathogenesis of CFS.