链脲佐菌素诱导大鼠糖尿病合并心肌缺血再灌注损伤模型的建立与评价

Establishment and Evaluation of Rats Model of Diabetic Myocardial Ischemia-reperfusion Injury Induced by Streptozotocin

目的建立稳定的大鼠糖尿病合并心肌缺血再灌注损伤模型,为干预实验提供平台。方法 45只健康雄性SD大鼠随机分为正常假手术组(Sham组)、糖尿病模型组(DM组)、糖尿病心肌缺血再灌注损伤模型组(DM+I/R组)。DM组和DM+I/R组一次性腹腔注射链脲佐菌素(STZ)60mg/kg建立糖尿病大鼠模型。DM+I/R组建模成功后采用结扎左冠状动脉前降支30min时进行再灌注120min方法制备缺血再灌注模型,Sham组和DM组仅在左冠状动脉前降支下穿线。于注射STZ后1周、2周、3周、4周观察大鼠空腹血糖、体重、尿量、饮水量及一般情况,用2,3,5-氯化三苯基四氮唑(TTC)测定心肌梗死面积,HE染色,光镜下观察心肌病理学损伤。结果与sham组比较,DM组和DM+I/R组腹腔注射STZ后空腹血糖值显著升高,体重显著下降,尿量和饮水量明显增加(P<0.05);与DM组比较,DM+I/R组心肌梗死面积明显增加(P<0.05),心肌病理学损伤加重。结论本实验方法制备的大鼠糖尿病合并心肌缺血再灌注损伤模型成功率较高,具有较好的稳定性,适用实验研究。

Objective To establish the diabetic myocardial ischemia reperfusion injury model in rats, to provide a platform for inter -vention experiment.Methods Forty five healthy male Sprague Dawley （ SD）rats were randomly divided into three group normalcontrol group （ Sham group） , model of diabetes （ DM group） , diabetes and myocardial ischemia reperfusion injury model group （ DM＋I/R group） .Myocardial ischemia was induced by 30 min occlusion of left anterior descending branch of coronary artery followed by 120min reperfusion .The rats of DM group and DM＋I/R group by intraperitoneal were injected Streptozotocin （ STZ）（ 60 mg/kg）to es -tablish diabetic rats model.After 1 week, 2 weeks, 3 weeks and 4 weeks, the injection of STZ to observe the rats fasting plasma glu -cose, body weight, urine volume, water quantity and the general situation.The myocardial infarction area was measured by tripheny -ltetrazolium chloride TTC.HE staining and myocardial pathology were observed under light damage.Results Compared with thesham group, the fasting blood glucose value in DM group and DM＋I/R group were significantly increased after intraperitoneal injec -tion of STZ, the weight was significantly reducted, and the urine volume and water quantity were increased significantly .Comparedwith DM group, the myocardial infarction area of DM＋I/R group was obviously increased, myocardial pathology injury aggravated.Conclusion： The experiment method of diabetic myocardial ischemia reperfusion injury in rats model of success rate is higher, hasgood stability, and applied research.