去窦弓神经对大鼠神经细胞线粒体Ca^(2+)和膜电位的影响

Effects of Sinoaortic Denervation on Mitochondrial Ca^(2+)and Membrane Potential of Nerve Cell in Rats

目的探讨去窦弓神经(SAD)对大鼠神经细胞线粒体Ca2+和膜电位的影响。方法选取SD大鼠50只随机分为对照组,假手术组,SAD30d组、SAD60d组、SAD90d组,采用火焰原子吸收法测线粒体Ca2+浓度,激光共聚焦显微镜检测膜电位。结果假手术组血压与对照组比较差异无统计学意义,SAD组与对照组比较24h舒张压和收缩压波动明显(P<0.01);SAD30d组、SAD60d组、SAD90d组与对照组及假手术组比较Ca2+浓度明显升高(P<0.01),膜电位明显降低(P<0.01);而且SAD组随时间延长Ca2+浓度逐渐升高(P<0.01),膜电位逐渐降低(P<0.01)。结论去窦弓神经后导致的血压波动,对脑细胞线粒体造成明显损害,且随时间延长日益严重,因此维持血压稳定至关重要。

Objective To explore the effects of sinoaortic denervation （ SAD） on mitochondrial Ca2＋ and membrane potential of nervecell in rats. Methods Fifty Sprague Dawley （ SD）rats were randomly divided into control group, sham operation group, 30 daySAD group, 60 day SAD group and 90 day SAD group. Mitochondrial membrane potential （ by Laser scanning confocal micro -scope）and level of Ca2＋（ by flame atomic absorption spectrometry）were measured and compared between the five groups.Results There were no differences in blood pressure between the sham operation group and the control group. Compared with the controlgroup, both systolic blood pressure and diastolic blood pressure in three SAD groups fluctuated obviously within 24 hours.The level ofCa2＋ of mitochondrion was elevated significantly（ P 〈0.01） and mitochondrial membrane potential was decreased significantly（ P 〈0.01） in the three SAD groups compared with that in the control group and sham operation group. And in the three SAD groups, thelevel of Ca2＋ of mitochondrionwas gradually increased and mitochondrial membrane potential was gradually decreased（ P 〈0.01） .Conclusion The fluctuation of blood pressure caused by sinoaortic denervation, which is a significant damage to the mitochondria ofbrain cells, and is becoming more and more serious with time.It is very important to maintain the stability of blood pressure.