摘要
从斑蝥(Lytta vesicatoria)体内分离得一株具有显著抗肝癌活性的真菌菌株,通过菌株形态与18S r DNA序列分析,鉴定为尖孢镰刀菌(Fusarium oxysporum,BM2).运用中压柱色谱和制备高效制备液相方法对BM2发酵液提取物乙酸乙酯部位的化学成分进行了分离纯化,利用现代波谱技术鉴定所分离化合物的结构,并采用MTT法对所得到的化合物进行了体外抗肝癌活性测试.结果表明:从发酵液提取物乙酸乙酯部位中分离得到的6个单体化合物,分别鉴定为麦角甾醇(1)、cyclo(R-Pro-S-Phe)(2)、(R)-2-羟基-3-苯基丙酸甲酯(3),4-氧代-乙酰丙酸(4),N-(4-氧代戊基)-乙酰胺(5),(R)-2-羟基-3-(4'-羟基苯基)丙酸甲酯(6).化合物2对2个肝癌细胞株Hep G2和SMMC-7721均有抑制作用,其IC50值分别为44.1和47.6μg/m L.
In this study, an endophytic fungus with appreciable anti-hepatomacarcinoma activity was isolated from Lytta vesicatoria and determined as Fusarium oxysporum ( BM2) , based on the fungus^ morphology and 18S r DNA sequence analysis. The isolation and purification of the ethyl acetate extract of BM2 fermentation was performed with medium-pressure column chromatography and preparative HPLC. The structures of the separated compounds were identified by modern spectroscopic techniques. In vitro anti-hepatomacarcinoma activity of the compounds was also evaluated using MTT method. Six pure compounds were obtained and their structures were elucidated as ergosterol (1),cyclo ( β-Pro-S-Phe) (2 ), methyl(β)-2-hydroxy-3-phenyl-propionate (3) , 4-oxopentanoic acid (4), N - (4-oxopentyl) -acetamide ( 5 ), and methyl (R) -2-hydroxy-3-(4r-hydroxyphenyl) propanoate (6) based on detailed spectral analysis. Compound 2 exhibited cytotoxic activity against HepG2 and SMMC-7721cell lines, with an IC50 value of 44. 1 and 47. 6 μg/mL, respectively.
出处
《中南民族大学学报(自然科学版)》
CAS
北大核心
2016年第3期42-47,共6页
Journal of South-Central University for Nationalities:Natural Science Edition
基金
国家自然科学基金资助项目(81573561)
中央高校基本科研业务费专项资金项目(GZY11073)
关键词
斑蝥
内生真菌
化学成分
抗肝癌活性
Lytta vesicatoria
Endophytic fungi
chemical constituents
anti-hepatomacarcinoma activity