深圳地区环境中创伤弧菌携带质粒分析及多位点序列遗传分型

Plasmid diversity and multilocus sequencing typing of Vibrio vulnificus isolated from the environment of Nanshan District,Shenzhen City

目的了解2010-2013年深圳市南山区环境中不同样品中分离的创伤弧菌质粒携带情况及基因的遗传多样性。方法采用聚合酶链反应技术检测深圳市南山区环境样品中创伤弧菌的6种质粒基因:seq25、seq51、Tra I、Tra I2、Tra D及Vep07。采用多位点序列分子分型方法进行分子分型及聚类分析。结果 50株创伤弧菌中,43株菌(43/50,86%)携带质粒,形成19种质粒图谱:20株(20/50,40%)同时存在毒力与结合质粒,13株(13/50,26%)仅存在毒力质粒,2株(2/50,4%)仅存在结合质粒。多位点序列分型方法将50株创伤弧菌,分为43个ST型。结论质粒广泛存在于深圳市南山区环境样品分离的创伤弧菌中,不同类型样本分离菌株携带质粒存在差异且具有遗传多样性。

Objective To investigate the plasmid diversity and genetic diversity of Vibrio vulnificus isolated from different envi- ronment samples in Nanshan District, Shenzhen City from 2010 to 2013. Methods Polymeras chain reaction was used to detect 6 kinds of plasmid genes of Vibrio vulnificus （including seq25, seq51, Tral, Tral2, TraD and Vep07） isolated from the environmental samples in Nanshan District, Shenzhen City. Vibrio vulnificus was collected to perform muhilocus sequencing typing （ MIST）, and MIST data were analyzed by the Bionnmerics software. Results Among the 50 strains of Vibrio vulnificus , 43 （86%） carried the plasmid, and 19 plasmid profiles presented 20 （20/50, 40%） strains possessed the virulence and conjugative plasmid, 13 （ 13/50, 26%） possessed the virulence plasmid and 2 （2/50, 4%） possessed the conjugative plasmid. 43 sequence types were found in 50 strains of Vibrio vulnificus by MLST. Conclusions Plasmids are widespread among Vibrio vulnificus spe- cies from the environment samples in Nanshan District, Shenzhen City. There exists diversity of plasmid profiles among Vibrio vulnificus strains isolated from different environment samples; moreover, Vibrio vulnificus strains also show genetic diversity.