碳点-荧光素荧光共振能量转移体系在阿司匹林测定中的研究与应用

Study on Carbon Dots-Fluorescein Fluorescence Resonance Energy Transfer System and Its Application in Aspirin Analysis

研究了经L-y半胱氨酸修饰后的碳点（CDs）-荧光素（FAM）荧光共振能量转移体系，并利用该体系建立了测定阿司匹林（ASP）的新方法。结果表明：在λex=330 nm下，于pH 7.0的Tris-HCl缓冲液中，CDs与FAM反应5 min后能发生有效的荧光共振能量转移，能量供体CDs将能量转移到受体FAM，使FAM的荧光显著增强，而ASP的加入可有效猝灭FAM的荧光，且ASP浓度在1.0~150.0 μg/mL范围内与体系的荧光猝灭值ΔIF呈良好的线性关系（r=0.999 6），基于此建立了测定ASP的新方法。在最佳实验条件下，方法的检出限达0.33 μg/mL（3δ/k,n=11），回收率为 97.1%~105.3%，相对标准偏差（RSD）不大于4.6%（n=6）。常见的无机离子以及与ASP同类型的药物对测定影响较小，方法的选择性较好。

A fluorescence resonance energy transfer（FRET）system between fluorescence carbon dots（CDs,donor）and fluorescein（FAM,acceptor）was constructed,and a novel method for the sensitive and selective determination of aspirin（ASP）was accordingly proposed.It was found that in pH 7.0 Tris-HCl buffer，effective energy transfer from CDs to FAM occurred,which resulted in a great enhancement of the fluorescence intensity of FAM.Upon the addition of ASP,fluorescence quenching of FAM occurred,and the change of FAM fluorescence intensity was in a good linearity（r=0.999 6）with ASP in the concentration range of 1.0-150.0 μg/mL.A novel fluorescence quenching platform for the determination of ASP was constructed.Under the optimum conditions,the detection limit of ASP for this method was 0.33 μg/mL（3δ/k,n=11）,and the spiked recoveries ranged from 97.1%to 105.3%with RSD（n=6）not more than 4.6%.The result showed that common relevant substance,cations and anions did not interfere with the detection of ASP.