摘要
建立了索氏提取-硅胶柱和凝胶柱净化/气相色谱-质谱联用(GC-MS)测定植物叶片中多环芳烃的分析方法。叶片中的多环芳烃经二氯甲烷-正己烷(体积比1∶1)索氏提取,提取液采用硅胶柱和凝胶柱两步净化(手填硅胶柱和GPC柱)后,进行GC-MS测定,根据保留时间和特征离子进行定性,内标法定量。方法检出限为0.025 1~5.80 ng/g,加标回收率为82.2%~130%,相对标准偏差不大于11%。该方法能有效去除植物叶片中的色素与油脂,适用于植物叶片中多组分多环芳烃的痕量分析,为测定植物组织内多环芳烃的含量提供了技术支撑。
A method was developed for the analysis of polycyclic aromatic hydrocarbons(PAHs) in plant leaves by gas chromatography-mass spectrometry(GC-MS) based on silica gel column and gel permeation chromatography(GPC) purification.The target analytes in leaf samples were extracted with dichloromethane-hexane(1∶1,by volume) solution,purified with silica gel column and GPC column,analyzed by GC-MS,and then quantified by the internal standard method.The average recoveries of 15 PAHs were in the range of 82.2%-130% with relative standard deviations(RSD) not more than 11%.The detection limit(LOD) of this method varied from 0.025 1 ng?g-1 to 5.80 ng?g-1.This method could effectively remove the pigment and oil in the plant leaves,suitable for the trace analysis of PAHs in leaf samples,and providing a technical support for the determination of content of PAHs in the plant tissues.
出处
《分析测试学报》
CAS
CSCD
北大核心
2016年第9期1185-1190,共6页
Journal of Instrumental Analysis
基金
林业公益性行业科研专项(201304301)
关键词
索氏提取
硅胶柱
凝胶柱
多环芳烃
气相色谱-质谱联用(GC-MS)
植物
soxhlet extraction
silica gel column
gel permeation chromatography(GPC)
polycyclic aromatic hydrocarbons(PAHs)
gas chromatography-mass spectrometry(GC-MS)
plant