硅胶柱与凝胶柱净化/气相色谱-质谱联用测定植物叶片中多环芳烃

Determination of Polycyclic Aromatic Hydrocarbons( PAHs) in Plant Leaf Samples by Silica Gel Column and Gel Column Purification / Gas Chromatography-Mass Spectrometry

建立了索氏提取-硅胶柱和凝胶柱净化/气相色谱-质谱联用（GC-MS）测定植物叶片中多环芳烃的分析方法。叶片中的多环芳烃经二氯甲烷-正己烷（体积比1∶1）索氏提取，提取液采用硅胶柱和凝胶柱两步净化（手填硅胶柱和GPC柱）后，进行GC-MS测定，根据保留时间和特征离子进行定性，内标法定量。方法检出限为0.025 1~5.80 ng/g，加标回收率为82.2%~130%，相对标准偏差不大于11%。该方法能有效去除植物叶片中的色素与油脂，适用于植物叶片中多组分多环芳烃的痕量分析，为测定植物组织内多环芳烃的含量提供了技术支撑。

A method was developed for the analysis of polycyclic aromatic hydrocarbons（PAHs） in plant leaves by gas chromatography-mass spectrometry（GC-MS） based on silica gel column and gel permeation chromatography（GPC） purification.The target analytes in leaf samples were extracted with dichloromethane-hexane（1∶1，by volume） solution，purified with silica gel column and GPC column，analyzed by GC-MS，and then quantified by the internal standard method.The average recoveries of 15 PAHs were in the range of 82.2%-130% with relative standard deviations（RSD） not more than 11%.The detection limit（LOD） of this method varied from 0.025 1 ng？g-1 to 5.80 ng？g-1.This method could effectively remove the pigment and oil in the plant leaves，suitable for the trace analysis of PAHs in leaf samples，and providing a technical support for the determination of content of PAHs in the plant tissues.