口腔癌患者尿液萘酚反应物的高效液相色谱荧光检测法筛查

Screening of Naphthol Reactant in Urine of Oral Cancer Patients by High Performance Liquid Chromatography with Fluorescence Detection

利用高效液相色谱荧光检测法(HPLC/FLD)对口腔癌患者尿液中的萘酚反应物(NNR)进行分离分析,通过对34例口腔癌患者和18例健康人尿液的NNR进行分析,比较了癌症患者及健康人尿液中的成分含量差异,提出了一种可用于口腔癌辅助诊断的新方法。结果表明,在健康人及口腔癌患者尿液的萘酚反应物(NNR)中可检出一种标记物,其激发波长为440 nm,发射波长为539 nm。该标记物含量在口腔癌患者中较低,而在健康人群中显著偏高,这与通常的鉴定结果不同。通过采用非参数检验(Mann-Whitney U检验),该标记物含量在健康人群与口腔癌症患者中存在显著性差异(P<0.01),其特异度为94.1%,敏感度为94.4%,ROC曲线下面积(AUC)为0.989。该模型具有较高的诊断价值,能够用于口腔癌的辅助鉴定。

A new aided diagnosis of oral cancer was developed for the separation and analysis of nitrosonaphthol reactant（NNR） of urine in oral cavity cancer patients by using high performance liquid chromatography with fluorescence detection（HPLC／FLD）,based on the analysis of urine NNR of 34 cases of oral cancer patients and 18 healthy people and their difference of composition content.The experimental results showed that a marker with excitation wavelength of 440 nm and emission wavelength of 539 nm,could be detected in urinary nitrosonaphthol reactant（NNR） from healthy people and patients with oral cavity cancer under the condition of this paper.The marker content was relatively low in oral cancer patients and significantly high in healthy persons,which is quite different from the usual test results.Significant differences were found in the matter content in healthy people and those with oral cancer patients（P〈0.01） using a non parametric test （Mann-Whitney U test）.Further data analysis showed that the specificity was 94.1%,the sensitivity was 94.4%,the area under the ROC curve（AUC） was 0.989.The model showed a high diagnostic value,and could be used for the auxilary identification of oral cancer.