复方金铃四逆四物失笑散治疗实验性原发性痛经的作用机制研究

Mechanisms of Fufang Shixiao Formula for Experimental Primary Dysmenorrhea

目的观察临床经验方复方金铃四逆四物失笑散(FFSXF)对原发性痛经模型大鼠子宫的前列腺素和外周血中β-内啡肽(β-EP)的作用,探讨其治疗痛经的作用机制。方法采用苯甲酸雌二醇联合缩宫素制备大鼠原发性痛经模型,以消炎痛、月月舒作对照药,根据FFSXF临床常用剂量,由人与大鼠体重换算后每天分别予FFSXF低、中、高剂量组2.5、5、10 g/kg浓度的FFSXF混悬液灌胃,采用ELISA对照考察药物对子宫组织前列腺素E2(PGE2)、前列腺素F2α(PGF2α)的变化,放射免疫法观察药物对其外周血液中β-EP含量的影响。结果与模型组比较,FFSXF高、中、低剂量组PGE2含量显著上升(P<0.01),PGF2α含量显著下降(P<0.05,P<0.01);FFSXF高、中剂量组β-EP含量显著上升(P<0.01)。结论 FFSXF能有效调节原发性痛经模型大鼠子宫组织中前列腺素水平,同时提高外周血液中的β-EP水平,增强内源性镇痛作用,可能是其治疗原发性痛经的作用机制。

Objective To observe the roles of Fufang Shixiao Formula（FFSXF） in regulating prostaglandin E2（PGE2）,prostaglandin F2 alpha（PGF2α）,and β-endorphin peptide（β-EP） in rats with primary dysmenorrhea,and to explore its mechanisms for treating primary dysmenorrhea.Methods Primary dysmenorrheal rat model was induced by Estradiol Benzoate combined oxytocin.Indomethacin and Yueyueshu were used as the controls.2.5,5.0,and 10.0 g/kg FFSXF（clinical commonly used dose of FFSXF calculated by converting human weight to rat weight）suspensions were administered to rats in low,middle,high dose FFSXF groups,respectively.Changes of PGE2 and PGF2α in uterus tissue were observed by ELISA.Its effect on β-EP in peripheral blood was observed by radioimmunoassay.Results Compared with the model group,PGE2 content significantly increased（P〈0.01）,and PGF2α content significantly decreased in the 3 FFSXF groups（P〈0.05,P〈0.01）,and β-EP content significantly increased（P〈0.01） in middle and high dose FFSXF groups.Conclusion FFSXF could effectively regulate prostaglandin level in uterus tissue of primary dysmenorrheal model rats,elevate β-EP content in peripheral blood,strengthen endogenous analgesic effects,which might be its mechanisms for treating primary dysmenorrhea.