复方附子多糖对肿瘤细胞糖基化作用的影响

Effects of Compound Aconitum Polysaccharide on Glycosylation of Tumor Cells

目的观察中药多糖对糖蛋白糖链合成的不同糖基转移酶的诱导或阻遏作用,从而发现中药多糖的作用靶点及其作用机制。方法对含有附子多糖的不同药物分组,以肿瘤生长抑制率来观察复方多糖体内抗肿瘤作用,观察中药多糖对肝癌SK-HEP-1细胞糖基转移酶以及肿瘤相关基因表达的影响,同时借助多聚乳糖胺特异性生物素标记凝集素进行流式细胞术检测细胞多聚乳糖胺表达水平变化。结果与模型组比较,各给药组平均瘤重均显著降低(P<0.01);与阿霉素组比较,3个复方组的平均瘤重无统计学意义(P>0.05)。分别加附子多糖与中药复方多糖,多聚乳糖胺表达水平降低。结论多糖复方配伍抗肿瘤效果基本与阿霉素效果一致,且3个复方配伍组随着熟附子粗多糖比例的增加,降低了多聚乳糖胺表达水平,抗肿瘤作用更加明显。

Objective To observe inducing or inhibiting effects of Chinese medicine（CM） polysaccharides on glycoprotein chain synthetized different glycosyltransferases,thus disclosing targets of CM polysaccharides and its mechanisms.Methods In vivo anti-tumor effects of CM polysaccharides were observed using the inhibiting rate of tumor growth by dividing different Aconihim containing groups.Effects of CM polysaccharides on liver cancer cell SK-HEP-1 glycosyltransferase and tumor related gene expressions were observed.Meanwhile,changes of polylactosamine expression were detected using flow cytometry（FCM） with polylactosamine specific biotin labeling lectin.Results Compared with the model group,the average tumor weight was significantly lower in each medication group（P〈0.01）.Compared with the adriamycin group,no significant difference in average tumor weight of the three compound groups（P〉 0.05）.The expression level of polylactosamine was reduced after adding Aconitum polysaccharide;and CM compound polysaccharides respectively.Conclusions Polysaccharide compound showed similar anti-tumor effect as that of adriamycin.Besides,polylactosamine expression level was reduced in the three compound groups along with increased prepared Aconitum polysaccharide,with more obvious anti-tumor effects shown.