针刺对支气管哮喘大鼠肺组织c-fos蛋白表达的影响

Effect of Acupuncture on c-fos Expression in the Lung Tissue of Bronchial Asthmatic Rats

目的探讨针刺对哮喘大鼠肺组织中c-fos蛋白表达的影响。方法将70只SPF级雄性SD大鼠随机分为空白组(A组)、哮喘模型组(B组)、空白针刺对照组(C组)、哮喘模型针刺对照组(D组)、哮喘模型针刺组(E组)、哮喘模型假针刺组(F组)和空白针刺组(G组),每组10只。各组分别给予相应干预后,用免疫组化、Western blot法检测大鼠肺组织c-fos蛋白的表达。结果免疫组化示A、C、G、E组c-fos蛋白表达呈阴性,B、D、F组呈弱阳性;Western blot检测示B组c-fos蛋白表达较A、E组升高(P<0.01);E组低于D、F组(P<0.05,P<0.01)。结论针刺可降低大鼠肺组织中c-fos蛋白表达以减轻炎症反应。

Objective To observe the effect of acupuncture on c-fos expression in the lung tissue of asthmatic rats.Methods Totally 70 SPF grade male SD rats were randomly divided into 7 groups,i.e.,the blank group（A）,the asthma model group（B）,the blank control group（C）,the asthma-model acupuncture control group（D）,the asthma model acupuncture group（E）,the asthma model sham-acupuncture group（F）,the blank acupuncture group（G）,10 rats in each group.Corresponding interventions were performed to each group.The protein expression of c-fos in lung tissue of rats was detected using immunohistochemistry and Western blot respectively.Results Immunohistochemistry showed negative expression of c-fos protein in Group A,C,G,and E,and weakly positive in Group B,D,and F.Results of Western blot showed the protein expression of c-fos was higher in Group B than in Group A and E（P〈0.01）.The protein expression of c-fos was lower in Group E than in Group D and F（P〈0.05 P〈0.01）.Conclusion Acupuncture could reduce the protein expression of c-fos in lung tissue,thus attenuating inflammation reaction.