RP-HPLC法测定犬血浆中奈诺沙星浓度

Determination of nemonoxacin in dog plasma by RP-HPLC

目的采用HPLC法测定比格犬血浆中喹诺酮类药物奈诺沙星的浓度。方法以莫西沙星作为内标，血浆样品经二氯甲烷萃取后，采用HPLC法进行测定，色谱柱为Welchrom DOS-C18 （200mm×6mm，5μm），流动相为乙腈－水（含0．2％三乙胺，磷酸调pH2．8）（22：78），检测波长292nm，流速1mL／min，柱温40℃。结果血浆中奈诺沙星的线性范围为0．1～12．0μg／mL（r=0．9996），平均方法回收率为101．25％，日内RSD〈8．0％，日间RSD〈8．5％。结论所用方法的专属性强、准确、灵敏、快速简便，适用于测定血浆中奈诺沙星的浓度。

Objective To establish an HPLC method for determination of nemonoxacin in beagles plasma. Methods Moxifloxacin was used as internal standard, the plasma samples were pretreated by dichloridimethane, and then analyzed by HPLC. The HPLC was performed on a Welchrom DOS-C18 column （200mm×4.6mm, 5μm） with UV detection at 292nm, using phosphoric acid solution （containing 0.2% triethylamine, adjusted to pH2.8 with phosphoric acid ） and acetonitile （78：22） as mobile phase at 1.0mL/min flow rate, And the column temperature was 40~C. Results The concentration of nemonoxacin was in good linearity in the range of 0.1-12.0pg/mL （r=0.9996）. The average recovery rate was 101.25%.The within-day and between-day RSD were less than 8.5%. Conclusion The method was simple, sensitive and accurate,. It can be used for determination ofnemonoxacin in plasm sample.