生脉注射液对脂多糖诱导大鼠炎症反应及血管内皮损伤的影响

Effects of Shengmai injection on LPS-induced inflammatory response and vascular endothelial cell injury in septic rats

目的:观察生脉注射液对脂多糖诱导的脓毒症大鼠炎症反应和血管内皮细胞损伤的影响。方法:将雄性Sprague-Dawley(SD)大鼠54只,随机分为对照组,脂多糖组和生脉治疗组,每组各18只。对照组腹腔注射生理盐水,脂多糖组和生脉治疗组腹腔注射脂多糖制备脓毒症大鼠模型,生脉治疗组实验前24h,注射脂多糖前1h分别静脉注射生脉注射液2ml/kg,制模成功后抽血检测血清白介素6(IL-6)和肿瘤坏死因子α(TNF-α),血清血管假性血友病因子(vWF)及血栓调节蛋白(TM)水平。结果:脂多糖组和生脉治疗组血清中IL-6、TNF-α、vWF和TM明显高于对照组(P<0.05);生脉治疗组IL-6、TNF-α、vWF、TM浓度较脂多糖组明显降低(P<0.05)。结论:生脉注射液可抑制脓毒症炎性因子的释放,对于脓毒症血管内皮细胞损伤具有一定的保护作用。

Objective：To investigate the effect of Shengmai injection on LPS-induced inflammatory response and vascular endothelial cell injury.Method：Fifty-four rats were randomly（random number）assigned into control group（n=18）,lipopolysaccharide（LPS）group（n=18）,Shengmai injection therapy group（n=18）.The sepsis model was prepared by injection of lipopolysaceharide（10 mg/kg）for LPS group and Shengmai therapy group.Rats in Shengmai therapy group were treated with Shengmai injection.ELISA assay was used to determine the level of tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,Plasma von Willebrand factor（vWF）and thrombomodulin（TM）.Result：The IL-6,TNF-α,vWF and TM were higher in LPS group and Shengmai injection treatment group than those in control group（P〈0.05）.The IL-6,TNF-α,vWF and TM were much lower in Shengmai injection treatment group than those in LPS group（P〈0.05）.Conclusion：Shengmai injection has effects on protection of endothelial cells by inhibiting the release of IL-6and TNF-αin septic rats.