期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

IGRP_(206-214)dtSCT四聚体偶联皂草素的制备及初步应用 被引量：1

The preparation and preliminary application of Saporin-Coupled dtSCT IGRP_(206-214) H-2K^d in NOD mice
原文传递
导出
摘要 以InsB15-23dtSCT原核表达载体为基础,制备dtSCT型IGRP206-214H-2Kd原核表达载体;将获取的融合蛋白IGRP206-214H-2Kd单体,生物素化制成IGRP206-214dtSCT四聚体,进一步将皂草素与IGRP206-214dtSCT四聚体偶联;流式检测皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育体系中IGRP206-214特异性细胞毒性T细胞(CTL)。结果表明:皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育后,IGRP206-214特异性CTL的频率与PBS对照组相比明显下降(P<0.001)。说明偶联皂草素的IGRP206-214dtSCT四聚体在体外能够靶向清除NOD鼠抗原特异性CTL。 ABSTRACT： On the basis of InsB15 23 dtSCT prokaryotic expression vector, dtSCT type of IGRP206-214 H-2Kd prokaryotic expression vector was eonstruet. The obtained fusion protein IGRP20G-214 H-2Kd monomer was biotinylated, then the IGRP206 214 dtSCT tetramer was coupled with saporin. The spleen lymphoeytes from NOD were co-cultured with saporin- Coupled dtSCT IGRP206-214 H-2Kd tetramer and the IGRP206- 214-speeific CTLs were detected by Flow Cytometry. Co-incu- bated NOD spleen lymphocytes together with Saporin Coupled dtSCT IGRP206- 214 H-2Kdtetramer, the IGRP206-214-specifie CTLs significantly decreased compared to the PBS control group （P（0. 001, n= 6）. These results showed that Saporin Coupled dtSCT IGRP206-214 H-2Kd tetramer can selectively clear NOD specific CTLs in vitro.
作者 王宇航 窦延 徐娅雯 马旻轩 李凯 潘兴元 钱莉 龚卫娟 季明春
机构地区 扬州大学医学院
出处 《扬州大学学报（农业与生命科学版）》 CAS 北大核心 2016年第2期12-16,共5页 Journal of Yangzhou University：Agricultural and Life Science Edition
基金 国家自然科学基金资助项目(30771955) 江苏省高校研究生科技创新计划项目(CXLX12-0939)
关键词 H-2Kd四聚体 CD8+T细胞 1型糖尿病 NOD鼠 H-2Kd tetramer CD8＋T cells type 1 diabetes mellitus NOD mice
分类号 R392.2 [医药卫生—免疫学]
  • 相关文献

参考文献17

  • 1CULINA S,BREZAR V,MALLONE R.Insulin and type I diabetes:immune connections [J].Eur J Endocrinol,2013,168(2):R19-R31.
  • 2ANDERSON M S,BLUESTONE J A.The NOD mouse:a model of immune dysregulation [J].Annu Rev Immu-nol,2005,23:447-485.
  • 3QIN H,TRUDEAU J D,REID G S,et al.Progression of spontaneous autoimmune diabetes is associated with a switch in the killing mechanism used by autoreactive CTL [J].Int Immunol,2004,16(11):1657-1662.
  • 4TRUDEAU J D,KELLY-SMITH C,VERCHERE C B,et al.Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood [J].The Journal of Clinical Investigation,2003,111(2):217-223.
  • 5潘兴元,梁锴,余鸣鸣,陈则东,窦延,季明春.膜表达型InsB_(15-23) H-2K^d dtSCT诱生小鼠特异性CTL细胞研究[J].扬州大学学报（农业与生命科学版）,2014,35(3):11-15. 被引量：2
  • 6胡小翠,孙丽君,潘少坤,罗红梅,方琳,沈玮,曹祥荣.重组hIL-24原核表达载体的构建及表达[J].扬州大学学报（农业与生命科学版）,2009,30(3):57-60. 被引量：3
  • 7潘兴元,蒋芳芹,梁锴,余鸣鸣,陈则东,窦延,季明春,焦新安.应用dtSCT技术制备低亲和力抗原肽InsB_(15-23)肽的MHCⅠ四聚体[J].免疫学杂志,2013,29(12):1074-1078. 被引量：2
  • 8LEHUEN A,DIANA J,ZACCONE P,et al.Immune cell crosstalk in type I diabetes [J].Nat Rev Immunol,2010,10(7):501-513.
  • 9KATZ J,BENOIST C,MATHIS D.Major histocompatibility complex class I molecules are required for the devel-opment of insulitis in non-obese diabetic mice [J].Eur J Immunol,1993,23(12):3358-3360.
  • 10YAGI H,MATSUMOTO M,KUNIMOTO K,et al.Analysis of the roles of CD4+ and CD8+ Tcells in autoim-mune diabetes of NOD mice using transfer to NOD athymic nude mice [J].Eur J Immunol,1992,22(9) : 2387-2393.

二级参考文献50

  • 1李湘鸣,罗方妮,陈春波,陈秀云,朱网娣.人雌激素受体cDNA在酵母细胞中的表达[J].扬州大学学报（农业与生命科学版）,2005,26(1):19-22. 被引量：9
  • 2瞿少刚,陈明,章晓联.白细胞介素-24基因重组表达载体的构建及原核表达[J].细胞与分子免疫学杂志,2005,21(3):273-275. 被引量：3
  • 3李慧忠,畅继武.可溶性MHC-肽四聚体技术及其应用进展[J].现代免疫学,2007,27(1):85-88. 被引量：5
  • 4Ahman JD, Moss PA, Goulder PJ, et al. Phenotypic analysis of antigen-specific T lymphocytes[J]. Science, 1996, 274(5284): 94-96.
  • 5Lybarger L, Yu YY, Miley MJ, et al. Enhanced immune presentation of a single-chain major histocompatihility complex class I molecule engineered to optimize linkage of a C-terminally extended peptide[J]. J Biol Chem, 2003, 278(29): 27105-27111.
  • 6Yu YY, Netuschil N, Lybarger L, et al. Cutting edge: single-chain trimers of MHC class I molecules form stable structures that potently stimulate antigen-specific T cells and B cells[J]. J Immunol, 2002, 168(7): 3145-3149.
  • 7Truscott SM, Lybarger L, Martinko JM, et al. Disulfide bond engineering to trap peptides in the MHC class I binding groove[J]. J Immunol, 2007, 178(10): 6280-6289.
  • 8Li L, Herndon JM, Truscott SM, et al. Engineering superior DNA vaccines: MHC class I single chain trimers bypass antigen processing and enhance the immune response to low affinity antigens[J]. Vaccine, 2010, 28(8): 1911-1918.
  • 9Trudeau JD, Kelly Smith C, Verchere CB, et al. Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood[J]. J Clin Invest, 2003, 111(2): 217-223.
  • 10Heckman KL, Pease LR. Gene splicing and mutagenesis byPCR-driven overlap extension[J]. Nat Protoc, 2007, 2(4): 924-932.

共引文献5

同被引文献16

  • 1李湘鸣,罗方妮,陈春波,陈秀云,朱网娣.人雌激素受体cDNA在酵母细胞中的表达[J].扬州大学学报（农业与生命科学版）,2005,26(1):19-22. 被引量：9
  • 2WUCHERPFENNIG K W,EISENBARTH G S.Type I diabetes [J].Nature Immunology,2001,54(2):2995-3001.
  • 3WONG F S,JR J C.The role of CD4 vs.CD8 T cells in IDDM [J].Journal of Autoimmunity,1999,13(3):290-295.
  • 4SERGEY N,HOWSON J M M,WALKER N M,et al.Localization of type I diabetes susceptibility to the MHC class I genes HLA-B and HLA-A [J].Nature,2007,450(7171):887-892.
  • 5BARRAL A M,THOMAS H E,LING E M,et al.SOCS-I protects from virally-induced CD8 T cell mediated type I diabetes [J].Journal of Autoimmunity,2006,27(3):166-173.
  • 6JINGUO W,SUE T,BINGYE H,et al.Autoantigen recognition is required for recruitment of IGRP(206-214)-au-toreactive CD8+ T cells but is dispensable for tolerance [J],Journal of Immunology,2012,189(6):2975-2984.
  • 7HYUN-JA K,JONATHAN C,SUTHERLAND R M,et al.Functional cytotoxic T lymphocytes against IGRP206-214 predict diabetes in the non-obese diabetic mouse [J].Immunology &-Cell Biology,2014,92(7):640-644.
  • 8BROPHY S E,HOLLER P D,KRANZ D M.A yeast display system for engineering functional peptide-MHC complexes [J].Journal of Immunological Methods,2003,272:235-246.
  • 9SUSAN F W,KHAI S L,GWEN S,et al.Activation of insulin-reactive CD8 T-cells for development of autoim-mune diabetes [J].Diabetes,2009,58(5):1156-1164.
  • 10DEGANO M,GARCIA K C,APOSTOLOPOULOS V,et al.A functional hot spot for antigen recognition in a superagonist TCR/MHC complex [J].Immunity,2000,12(3):251-261.

引证文献1

二级引证文献2

扬州大学学报（农业与生命科学版）
2016年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部