人参皂苷Rg3对宫颈癌U14细胞凋亡水平影响的实验研究

Effect of ginsenoside Rg3 on apoptosis of cervical cancer U14 cells: an experimental research

目的 探讨人参皂苷Rg3对宫颈癌U14细胞凋亡水平的影响。方法 10%小牛血清的DMEM营养液培养宫颈癌U14细胞,待细胞铺满培养板底80%~90%时分为对照组、Rg3低剂量组（20μg/ml）、Rg3中剂量组（40μg/ml）、Rg3高剂量组（80μg/ml）和环磷酰胺阳性药组（50μg/ml）开始加药,培养24 h后终止培养。通过CCK-8法检测细胞增殖活性,倒置显微镜观察细胞生长状态及免疫细胞化学法检测细胞凋亡状况。结果 倒置显微镜观察可见随剂量增大U14细胞排列逐渐松散、萎缩,细胞漂浮死亡数量增多;CCK-8法检测显示Rg3低、中、高剂量组及阳性药组对U14细胞的增殖活性均有抑制作用,免疫细胞化学法显示Rg3低、中、高剂量组均能促进细胞凋亡,组间差异均有统计学意义（P〈0.01）,且阳性药组与高剂量组差异无统计学意义（P〉0.05）。结论 Rg3能明显抑制U14细胞生长,通过下调Survivin和Bcl-2基因、上调Caspase-3和Bax基因诱导其凋亡,从而发挥抑瘤作用。

Objective To explore the effect of ginsenoside Rg3 on apoptosis of cervical cancer U14 cells. Methods Cervical cancer U14 cells was cultured by 10% calf serum DMEM nutrient solution, until the cells were confluent culture of 80% -90% Of the bottom plate, the cells were divided into control group, Rg3 low dose group （20 μg/ml）, Rg3 medium dose group （40 μg/ml）, Rg3 high dose group （80 μg/ml）, and cyclophosphamide positive drug group （50 μg/ml）, culture was terminated after 24 hours, CCK-8 method was used to detect activity of cell proliferation, inverted microscope was used to observe cell growth state, immunocytochemistry was used to detect cell apoptosis. Results Inverted microscope observation showed that with the increase of the dose, cervical cancer U14 cells were gradually loose and atrophic, and the number of floating dead cells increased. CCK-8 assay showed that cervical cancer U14 cells in Rg3 low dose group, Rg3 medium dose group, Rg3 high dose group, and cyclophosphamide positive drug group were inhibited. Immunocytochemistry showed that apoptosis of cervical cancer U14 ceils was promoted in Rg3 low dose group, Rg3 medium dose group, and Rg3 high dose group, there was statistically significant difference （ P〈0. 01 ）, but there was no statistically significant difference between cyclophosphamide positive drug group and Rg3 high dose group （P〉0. 05） . Conclusion Rg3 can significantly inhibit the growth of cervical cancer U14 cells, apop- tosis of cervical cancer U14 cells is induced by down-regulation of Survivin gene and Bcl-2 gene, up-regulation of Caspase-3 gene and Bax gene to play a role in tumor inhibition.