基于UHPLC-LTQ-Orbitrap-MS/MS技术分析枳实中的化学成分

Analysis of chemical constituents from Citrus aurantium by UHPLC-LTQ-Orbitrap-MS / MS

应用超高效液相色谱-线性离子阱轨道阱串联质谱联用技术(UHPLC-LTQ-Orbitrap-MS/MS)对枳实中的化学成分进行定性分析。以75%甲醇作为提取液制备供试液,色谱分离选用Thermo Hypersil BDS反相柱(2.1 mm×150 mm,2.4μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱,电喷雾线性离子阱轨道阱质谱(ESI-LTQ-Orbitrap-MS/MS)正离子模式下进行检测,根据化合物的精确相对分子质量和碎片离子信息进行结构鉴定。结果从枳实中共检测到27个化合物,包含了14个黄酮类化合物,7个香豆素类化合物,5个柠檬苦素类化合物和1个生物碱类化合物。该文结果为进一步研究和利用中药枳实提供了参考。

In this study, a ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrome- try （UHPLC-LTQ-Orbitrap-MS/MS） method was developed to analyze the chemical components in Citrus aurantium. C. aurantium were extracted with 75% methanol, and we applied a Thermo Scientific BDS Hypersil C^scolumn （2. 1 mm × 150 mm, 2.4 μm） to UHPLC analysis with acetonitrile-water （containing 0. 1% formic acid） in gradient as mobile phase. Elutes were then detected by ESI- LTQ-Orbitrap-MS/MS. A total of 27 components were identified, including fourteen flavonoids, seven coumarins, five limonoids and one alkaloid. This study showed an insight into the composition of C. aurantium, which could provide theoretical foundation for further study and utilization of the medicinal resources.