SPME-GC-MS法分析聚筛蕊挥发性化学成分

Analysis of Volatile Components in Cladia aggregata by SPME-GC-MS

目的：采用固相微萃取．气相色谱．质谱联用法分析聚筛蕊挥发性化学成分。方法：色谱条件：色谱柱为ZB-5 MSI5％苯．95％二甲基聚硅氧烷弹性石英毛细管柱，程序升温[以5℃／min升温至220℃（保持2min）]，检测器为氢火焰离子化检测器，载气为高纯氦（纯度：99．999％），柱流速为1．0ml／min，汽化室温度为250℃，不分流进样。质谱条件：离子源为电子电离源，电离电压为70ev，接口温度为28（1℃，离子源温度为23（1℃，四极杆温度为150℃，发射电流为34．6μA，倍增器电压为l5（12V，溶剂延迟时间为1．5min，质量范围为30--500amu。结果：从聚筛蕊中共分离、鉴定出19种挥发性化学成分，主要为烯烃和醛、醇、酮、呋喃等含氧挥发性化学成分，相对百分含量占总离子流图中所有色谱峰的63．55％，其中含量较高的是2，4．辛二烯（29．66％）、己醛（5．93％）、3-甲基-1，4-庚二烯（5．45％）、呋喃（4．65％）。结论：该研究基本明确了聚筛蕊主要挥发性化学成分，为进一步认识聚筛蕊提供了-定科学依据。

OBJECTIVE： To analyze the volatile components of Cladia aggregate by SPME-GC-MS. METHODS： The column was ZB-5 MSI 5% benzene-95% dimethylpolysiloxane fused silica capillary column, programmed temperature was heated from 5 ℃/min to 220℃（maintaining 2 min）, detector was flame ionization detector, carrier gas was high purity He （99.999%）, the column flow rate was 1.0 ml/min, vaporization room temperature was 250℃, splitless injection. MS conditions： ion source was electron ionization source, ionizing voltage was 70 eV, interface temperature was 280 ℃, ion source temperature was 230℃, quadrupole temperature was 150℃, emission current was 34.6 ktA, multiplier voltage was 1 502 V, solvent delay time was 1.5 min, and quality range was 30-500 amu. RESULTS： 19 volatile chemical components were separated and identified from C. aggregate, mainly olefins an aldehydes, alcohols, ketones, furans oxygenated volatile chemical components, relative contents accounted for 63.55% to all chromatographic peaks in total ion chromatogram, in which, 2,4-octadiene （29.66%）, hexanal （5.93%）, 3-meth- yl-i, 4-heptadiene （5.45%） and furan （4.65%） showed the highest contents. CONCLUSIONS ： The study has basically made clear the main volatile components in C. aggregate, which provide certain scientific basis for further exploring C. aggregate.