快速溶剂萃取-HPLC法测定骨碎补中柚皮苷的含量

Determination of Naringin in Davallia mariesii by Accelerated Solvent Extration-HPLC

目的：为骨碎补中柚皮苷的提取及测定开辟新的途径。方法：采用ASE350型快速溶剂萃取系统提取骨碎补中的柚皮苷；以高效液相色谱法测定骨碎补中柚皮苷的含量。色谱柱为Kinetex XB—C18，流动相为甲醇-5％乙酸（25：75，V/V，流速为0．8ml／min，检测波长为283nm，柱温为40℃，进样量为10μl。结果：柚皮苷的进样量线性范围为0．073O～0.7300μg（r=0．9999））；定量限为0．73μg，检测限为0．022μg；精密度、稳定性、重复性试验的RSD〈2％；加样回收率为98．92％～100．85％（RSD=0．72％，n=6）。结论：该方法简便、准确、专属性强，可用于59,q定骨碎补中柚皮苷的含量。

OBJECTIVE： To develop a new way for the extration and determination of naringin in Davallia mariesii. METH- ODS： Naringin in D. mariesii was extracted by ASE350 accelerated solvent extraction system; HPLC was performed for the con- tent determination of naringin in D. mariesii, the column was Kinetex XB-C18 with mobile phase of methanol-5% acetic acid（25：75, V/V） at a flow rate of 0.8 ml/min, detection wavelength was 283 nm, column temperature was 40 ℃, and injection volume was 10 μl. RESULTS： The linear range of naringin was 0.073 0-0.730 0 μg（r=0.999 9） ; the limit of quantitation was 0.73μg, the limit of detection was 0.022μg; RSDs of precision, stability and reproducibility tests were lower than 2% ; recovery was 98.92%-100.85% （RSD=0.72% ,n=6）. CONCLUSIONS： The method is simple, accurate and specific, and can be used for the content determination of naringin in D. Mariesii.