水胺硫磷对鲫鱼肝微粒体酶CYP450和CYP1A1活性的影响

Effects of Isocarbophos on Activities of CYP450 and CYP1A1 Enzymes in Liver Microsome of Carassius auratus

[目的]观察鲫鱼静态暴露水胺硫磷对肝微粒体CYP450酶系活性的影响,探讨水胺硫磷污染水体的生物学效应。[方法]将鲫鱼分为5.0、10.0、15.0、20.0 mg/L 4个染毒组和1个对照组,进行水胺硫磷静态暴露染毒,在染毒24、48、72、96 h时动态监测鲫鱼肝微粒体CYP450酶及其亚酶CYP1A1的活性,同时用气相色谱法检测试验液中水胺硫磷水平,分析与肝CYP450酶及CYP1A1活性的相关性。[结果]当染毒24 h时,与对照组相比,各染毒组肝微粒体总CYP450酶活性无显著变化,20.0 mg/L组CYP1A1酶活性高于对照组(P<0.05);染毒48 h后所有染毒组总CYP450酶及CYP1A1酶活性较对照组均升高(P<0.01);当染毒96 h时,20.0 mg/L组总CYP450及CYP1A1酶活性分别是对照组的1.29倍和1.54倍。染毒24 h时试验液中水胺硫磷残留量与总CYP450酶及CYP1A1活性无相关关系(P>0.05),但在染毒48 h后就出现了显著的正相关关系,相关系数r均大于0.9(均P<0.05)。[结论]水胺硫磷暴露会引起鲫鱼肝CYP450酶及其亚酶CYP1A1的活性增加,且在暴露48 h以后水体中水胺硫磷的含量和CYP450酶及其亚酶CYP1A1的活性呈正相关关系。

[Objective] To observe the activities of hepatic CYP450 and CYP1A1 enzymes in the liver of Carassius auratus statically exposed to water polluted with isocarbophos and to assess the biological effects of isocarbophos on water body.[Methods] All Carassius auratus samples were divided into four experiment groups （concentration of isocarbophos： 5.0, 10.0, 15.0, and 20.0 mg/L） and one control group. The four experiment groups were then statically exposed to designed isocarbophos polluted water. After 24, 48, 72, and 96 h, the activities of CYP450 and CYP1A1 enzymes in liver microsome of Carassius auratus were measured. Meanwhile, the concentrations of isocarbophos in water samples were detected by gas chromatography, and the correlation between the concentrations of isocarbophos and the activities of CYP450 and CYP1A1 were evaluated. [Results] Within 24 h of exposure, compared with the control group, the liver total CYP450 enzyme activity in experiment group did not change, but the CYP1A1 enzyme activity in the 20.0 mg/L experiment group was higher than that in the control group （P 〈 0.05）. After 48 h of exposure, compared with the control group, the activities of total CYP450 and CYP1A1 enzymes in all experiment groups were obviously increased （P 〈 0.01）. After 96 h of exposure, the total CYP450 enzyme activity and the CYP1A1 enzyme activity of the 20.0 mg/L experiment group were 1.29 times and 1.54 times respectively higher than the corresponding indices of the control group. After 24 h of exposure, there were no correlations between the residues of isocarbophos and the activities of total CYP450 and CYP1A1 enzymes （P 〉 0.05）, but after 48 h of exposure, there existed positive correlations and the correlation coefficients were all over 0.9 （Ps 〈 0.05）. [Conclusion] Isocarbophos exposure could cause increased activities of CYP450 and CYP1A1 enzymes in Carassius auratus livers. After 48 h of exposure, isocarbophos concentrations in water and the activities of CYP450 and CYP1A1 enzymes are positively correlated.