分子标记转育3个基因改良空育131稻瘟病抗性

Transmitted Three Genes by Marker-assisted Selection to Improve Rice Blast Resistance of Kongyu131

为改良空育131的稻瘟病抗性,通过杂交和分子标记辅助选择技术将中金23的Pi-1、Pi-2和Pi-33稻瘟病抗性基因导入到受体亲本空育131中,并通过连续回交结合农艺性状筛选,获得了28个含有不同抗稻瘟病基因的空育131导入系,其中同时含有Pi-1、Pi-2和Pi-33这3个抗性基因的株系k165、k166和k191对稻瘟病的抗病频率均达到了98.4%,明显高于携带1个和2个抗性基因的株系,且k165的综合农艺性状已接近空育131,表明通过分子标记辅助选择聚合稻瘟病抗性基因是开展品种抗性定向改良的有效方法之一。

To improve the blast resistance of Kongyu 131,with backcrossing and molecular marker assisted selection（ MAS） technology,three rice blast resistance genes（ Pi- 1,Pi- 2 and Pi- 33） from indica variety Zhongjin 23 were introgressed into Kongyu 131. Through continuous backcross combined with agronomic traits selection,28 introgression lines of Kongyu 131 with different rice blast resistance genes were obtained. Among them,k165、k166 and k191 carring all three blast resistance genes（ Pi- 1,Pi- 2 and Pi- 33） possessed 98. 4% resistance frequency to different blast race,which significantly higher than that carrying 1 or 2 blast resistance gene lines,and the comprehensive agronomic traits of k165 line were similar to that of Kongyu 131. That suggested blast resistance gene pyramiding by molecular marker assisted selection is one of the effective approaches for directional improving blast of resistance of rice varieties.