雷公藤红素抑制舌鳞癌细胞和人单核/巨噬细胞分泌血管内皮生长因子实验研究

Effect of Tripterine on Vascular Endothelial Growth Factor Secretion of Squamous Cell Carcinoma and Monocyte/macrophages

目的探讨雷公藤红素(Tri)对人单核/巨噬细胞(THP-1细胞)、舌鳞癌细胞(Tca8113细胞)分泌血管内皮生长因子(VEGF)、血管内皮生长因子-C(VEGF-C)的影响。方法在酸性微环境中,加入不同浓度的Tri,分别与Tca8113细胞、THP-1细胞+Tca8113细胞、THP-1细胞一起培养72h,ELISA法检测细胞上清液中VEGF、VEGF-C含量,分析Tri对这两种生长因子的影响。结果随着Tri浓度以0、5、10、20、50μg/m L逐渐增高,各组的VEGF、VEGF-C值逐渐降低,且均与Tri浓度成负相关(P均<0.001)。VEGF值:THP-1组分别为(57.41±2.13)、(53.12±1.23)、(46.43±2.11)、(44.24±1.14)、(38.15±0.93)μmol/L;Tca8113组分别为(61.45±2.73)、(58.14±1.63)、(55.33±2.15)、(50.22±1.66)、(41.11±1.45)μmol/L;THP-1+Tca8113组分别为(56.72±2.13)、(54.22±1.23)、(47.42±2.14)、(43.52±1.16)、(39.12±0.83)μmol/L。VEGF-C值:THP-1组分别为(61.25±1.13)、(55.34±2.11)、(51.23±1.30)、(48.22±2.13)、(45.21±1.62)μmol/L;Tca8113组分别为(61.33±2.16)、(57.43±2.35)、(53.12±2.62)、(49.43±2.34)、(43.13±1.65)μmol/L;THP-1+Tca8113组分别为(60.35±2.13)、(55.74±2.15)、(50.13±1.61)、(47.42±2.14)、(40.11±1.55)μmol/L。THP-1+Tca8113细胞中各Tri剂量组VEGF、VEGF-C水平与THP-1细胞中各Tri剂量组的VEGF、VEGF-C水平相近,差异无统计学意义(P>0.05),但均低于Tca8113细胞中各Tri各剂量组VEGF、VEGF-C水平,差异有统计学意义(P<0.05)。结论随Tri浓度的增加,Tri对THP-1和Tca8113细胞分泌VEGF、VEGF-C因子的抑制作用逐渐增强,THP-1细胞可提高Tri对VEGF、VEGF-C的抑制作用,有助于抑制舌癌的生长和转移。

Objective To investigate the effect of tripterine on vascular endothelial growth facor （VEGF） and VEGF-C secretion of monocyte/macrophages （THP-1 cells）. Methods Oral squamous cell carcinoma cells （Tca8113 cells） and THP-1 cells were cultured with different concentrations of tripterine separately or together for 72 h in vitro, then VEGF and VEGF-C contents in supernatants were detected with ELISA method. Results As the concentration of tripterine increased from 0, 5, 10, 20 to 50 μg/mL, the contents of VEGF and VEGF-C de-creased gradually in all groups, which indicated a negative relation to the dose of tripterine （all P〈0.001）. The re-sults of VEGF contents in THP-1, Tca8113, and THP-1＋Tca8113 groups were as follows： THP-1： 57.41 ±2.13, 53.1±1.23, 46.43±2.11, 44.24±1.14, 38.15±0.93 μmol/L; Tca8113： 61.45±2.73, 58.14±1.63, 55.33±2.15, 50.22±1.66, 41.11±1.45 μmol/L; THP-1＋Tca8113： 56.72±2.13, 54.22±1.23, 47.42±2.14, 43.52±1.16, 39.12±0.83 μmol/L. The re-sults of VEGF-C contents in THP-1, Tca8113, THP-1＋Tca8113 groups were as follows： THP-1： 61.25 ±1.13,55.34 ±2.11, 51.23 ±1.30, 48.22 ±2.13, 45.21 ±1.62 μmol/L; Tca8113： 61.33 ±2.16, 57.43 ±2.35, 53.12 ±2.62, 49.43 ± 2.34, 43.13±1.65 μmol/L; THP-1＋Tca8113： 60.35±2.13, 55.74±2.15, 50.13±1.61, 47.42±2.14, 40.11±1.55 μmol/L）. The contents of VEGF and VEGF-C in THP-1＋Tca8113 groups were not significant different from those in THP-1 groups at each tripterine concentration （P〉0.05）, but significantly lower than those in Tca8113 groups （P〈0.05）. Con-clusion Tripterine can more inhibit VEGF and VEGF-C secretion of THP-1 cells and Tca8113 cells as its con-centration increases. THP-1 may enhance the inhibition of tripterine on VEGF and VEGF-C, which may result in suppressing the growth and metastasis of oral squamous cell carcinoma.