沙田柚热激蛋白HSP90基因的鉴定分析

Identification and Analysis of HSP90 Gene in Citrus grandis var.Shatianyu

为探明沙田柚自交不亲和分子机理,以沙田柚(Citrus grandis var.Shatianyu Hort)自交和异交花柱为材料,对其进行转录组测序;通过差异分析获得相关基因,并研究其理化性质。结果表明:获得的Hsp90基因全长2 602bp(GenBank登录号:KU517851),开放阅读框(ORF)全长为2 100bp,编码699个氨基酸,编码的蛋白质分子量为80.55KDa,理论等电点为5.01,含有1个与HSP90超家族(HSP90superfamily)相同的保守结构域。沙田柚Hsp90蛋白氨基酸的同源性与甜橙(XM_006490568)、克莱门柚(XM_006421973)亲缘关系较近,同源性高达99%,属同一进化分支。

The self-pollinated and cross-pollinated style of C.grandis var.Shatianyu were used as test materials and based on the successful transcriptome sequencing technology,the authors identified a Hsp90 gene to explore the molecular mechanism of self-incompatibility.Results：Hsp90 gene was 2602 bp（GenBank accession number：KU517851）in length with an open reading frame（ORF）of 2100 bp,encoding 699 amino acids with deduced molecular weight of 80.55 KDa,and theoretical pI value of 5.01,and contained a HSP90 superfamily conserved domain.The homology analysis indicated that the Hsp90 protein shared 99% homology with that of Citrus sinensis（XM_006490568）and Citrus clementina（XM_006421973）.Phylogenetic analysis revealed that Hsp90 gene showed closer kinship with that of C.sinensis and C.clementina,indicating that they belong to the same evolutionary branch.