养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury

目的对养心氏片防治慢性缺血性心力衰竭和急性心肌缺血再灌注损伤作用进行实验研究,为其临床应用提供理论依据。方法采用冠状动脉结扎法构建慢性缺血性心力衰竭大鼠模型及急性心肌缺血再灌注小鼠心梗模型,随机分为假手术组、模型组、阳性药组、以及养心氏片低、中、高剂量组,通过预给药和造模后给药,检测大鼠超声心电图、ACE、ACD及小鼠心肌梗死面积、CK、LDH、SOD、相关因子,并观察心脏病理学切片的形态学变化。结果与假手术组相比,模型组的生化指标差异具有统计学意义(P<0.001);与模型组相比,阳性药组、高剂量养心氏片组生化指标差异具有统计学意义(P<0.001,P<0.01,P<0.05),且能显著减少心肌梗死面积(P<0.01,P<0.05),明显改善心肌细胞水肿程度,减少细胞浸润。结论养心氏片对慢性缺血性心力衰竭、急性心肌缺血再灌注损伤具有明显的预防和治疗作用。

Objective Experimental study on effects of Yangxinshi tablets in the prevention and treatment of chronic is-chemic heart failure and acute myocardial ischemia reperfusion injury in order to provide a theoretical basis for its clinical appli-cation .Method Rat model of chronic ischemic heart failure and acute ischemia reperfusion mouse model of myocardial infarc-tion were constructed through coronary artery ligation .Those animals were randomized to sham operation group ,model group , positive drug group ,high Yangxinshi tablet dose group ,middle Yangxinshi tablet dose group ,low Yangxinshi tablet dose group .Rat electrocardiogram ,ultrasound ,ACE ,ACD ,TNF-a ,mouse myocardial infarction area ,CK ,LDH ,SOD and relat-ed factors were recorded during pre-dosing and post-dosing after modeling .Morphological changes were observed with cardiac pathological section .Results The biochemical indicators in model group were significantly different from sham operation group with statistical significance （P〈0 .001） .Compared to the model group ,positive drug group and high Yangxinshi tablet dose group exhibited biochemical differences with statistical significance （P〈0 .001 ,P〈0 .01 ,P〈0 .05） ,significant myocardial in-farction area reduction （P〈0 .01 ,P〈0 .05） ,improvement in myocardial cells and reduction in the cell infiltration .Conclusion Yangxinshi tablets have preventive and therapeutic effects on chronic ischemic heart failure and acute ischemia reperfusion injury .