花刺参中三个新的三萜皂苷类化合物

Three new triterpene glycosides from Stichopusvariegatus

目的研究花刺参体内的活性皂苷类成分。方法运用硅胶、Sephadex LH-20柱色谱等方法进行分离纯化,利用IR,1 H、13 C NMR,MS等光谱技术鉴定其化学结构。结果从花刺参中分离并鉴定出3个化合物,分别为3-O-[3-O-甲基-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃木糖-(1→3)-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃木糖]-海参烷-7,24-二烯-3β,23S-二醇,命名为variegatuside C;3-O-{3-O-甲基-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃木糖-(1→4)-β-D-吡喃葡萄糖-(1→2)[β-D-吡喃葡萄糖-(1→4)]-β-D-吡喃木糖}-海参烷-8-烯-3β,23S-二醇,命名为variegatuside D;3-O-{3-O-甲基-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃木糖-(1→4)-β-D-吡喃葡萄糖-(1→2)[3-O-甲基-β-D-吡喃葡萄糖-(1→3)-β-D-吡喃葡萄糖-(1→4)]-β-D-吡喃木糖}-海参烷-9-烯-3β,23S-二醇,命名为variegatuside E。结论 3个化合物均为新化合物。

Objective To study the chemical constituents from the Stichopus variegatus .Method The compounds were isolated and purified by silica gel column chromatography ,Sephadex LH-20 gel filtration and recrystallization .The structures of these compounds were identified through chemical reactions and spectral analyses ,such as IR ,^1H ,^13C NMR ,MS .Results Three compounds were identified as 3-O-[3-O-methyl-β-D-glucopyranosyl （1→3）-β-D-xylopyranosyl （1→3）-β-D-glucopyranosyl （1→2）-β-D-xylopyranosyl]-holosta-7 ,24-diene-3β,23S-diol ,named as variegatuside C ;3-O-{3-O-methyl-β-D-glucopy-ranosyl（1→3）-β-D-xylopyranosyl（1 → 4）-β-D-glucopyranosyl （1 → 2）[β-D-glucopyranosyl （1 → 4）]-β-D-xylopyranosyl-holosta-8-ene-3β, 23S-diol ,named as variegatuside D ;3-O-{3-O-methyl-β-D-glucopyranosyl（1→3）-β-D-xylopyranosyl（1→4）-β-D-glucopyranosyl （1→ 2 ） [3-O-methyl-β-D-glucopyranosyl （1 → 3 ）-β-D-glucopyranosyl （1 → 4 ）]-β-D-xylopyranosyl}-holosta-9-ene-3β,23S-diol , named as variegatuside E .Conclusion All three compounds are new compounds .