TAp63在胰腺癌中的表达及意义

TAp63 expression in pancreatic cancer and its significance

目的:探讨TAp63在胰腺癌中的表达及其意义。方法:分别real-time PCR和Western blot检测TAp63 m RNA与蛋白在21例胰腺癌及其配对癌旁组织、3种胰腺癌细胞(PANC-1、CFPAC-1、Bx PC3)及正常胰腺细胞(HPDE6-C7)中的表达;用TAp63-si RNA转染PANC-1细胞后,观察TAp63 m RNA与蛋白表达的变化,并用MTT和Brd U法检测转染后PANC-1细胞的增值情况。结果:胰腺癌组织中TAp63 m RNA表达量明显低于癌旁正常胰腺组织(t=2.572,P=0.0158);TAp63m RNA与蛋白在3种胰腺癌细胞株中的表达量均明显低于正常胰腺HPDE6-C7细胞(均P<0.05)。与未处理的PANC-1细胞比较,转染TAp63-si RNA后的PANC-1细胞TAp63 m RNA与蛋白明显降低,但细胞增殖与DNA合成能力均明显增强(均P<0.05)。结论:TAp63在胰腺癌组织与细胞中表达降低,且表达量越低,细胞生长能力越强。

Objective： To investigate TAp63 expression in pancreatic cancer and its signiifcance. Methods： hTe mRNA and protein expressions of TAp63 in 21 paired specimens of pancreatic cancer tissues and their adjacent normal pancreatic tissues, as well as three different pancreatic cancer cell lines （PANC-1, CFPAC-1 and BxPC3） and normal pancreatic cell line （HPDE6-C7） were determined by real-time PCR and Western blot, respectively. PANC-1 cells were transfected with TAp63-siRNA, and then, the changes in mRNA and protein expressions of TAp63 were observed and the proliferation of PANC-1 cells after transfection was detected by MTT and BrdU assay, respectively. Results： hTe TAp63 mRNA expression level in pancreatic cancer tissue was signiifcantly lower than that in their adjacent normal pancreatic tissues （t=2.572,P=0.0158）; both mRNA and protein expression levels of TAp63 were signiifcantly lower in all the three types of pancreatic cancer cell lines than those in HPDE6-C7 cells （allP〈0.05）. In PANC-1 cells transfected with TAp63-siRNA compared with untreated PANC-1 cells, both the abilities of＆amp;nbsp;proliferation and DNA synthesis were signiifcantly increased （allP〈0.05）. Conclusion： TAp63 expression is down-regulated in pancreatic cancer tissue and cells, and the lower the level of TAp63 expression, the stronger is the growth ability of the cells.