摘要
[目的]优化黄芪药材中黄芪甲苷提取方法。[方法]采用单因素试验对提取方式、提取溶剂、提取次数进行考察,采用HPLCELSD法测定黄芪甲苷含量。[结果]黄芪药材中黄芪甲苷最优提取方法为每次用50倍的50%甲醇加热回流提取2次,每次1 h。[结论]该试验所建立的方法操作简单、准确可靠、重现性好,为黄芪饮片及制剂中黄芪甲苷的提取及含量测定提供参考。
[Objective] The aim was to improve the extraction method of astragaloside IV from Astragali Radix.[Method] Three factors inclu-ding extraction method, extraction solvent and extraction times were studied through single-factor test.HPLC-ELSD method was adopted to ana-lyze the determination results of astragaloside IV.[Result] The best extraction method of astragaloside IV in Astragali Radix was as follows：50 times of 50% methanol was added, heated reflux extraction was adopted for two times with 1 hours for each extraction.[ Conclusion] The im-proved method is simple, accurate and reproducible, which can supply a reference for extraction and determination of Astragali Radix.
出处
《安徽农业科学》
CAS
2016年第22期125-127,共3页
Journal of Anhui Agricultural Sciences
关键词
黄芪
黄芪甲苷
提取方法
优化
Astragali Radix
Astragaloside IV
Extraction method
Optimization
