牙科用微弧氧化后锆基非晶合金的组织相容性研究

Histocompatibility evaluation of Zr-based bulk metallic glass with micro-arc oxidation for dental restoration

目的通过进行口腔黏膜刺激实验和皮下植入实验,研究微弧氧化后锆基非晶合金的组织相容性。方法实验参照ISO 10993-6:1994标准,分别将微弧氧化前后的锆基非晶合金和对照用的钛合金制备成直径5 mm,厚0.5 mm的圆片样品和直径1.5 mm,长5 mm的柱状样品,进行口腔黏膜刺激实验和皮下植入实验。结果口腔黏膜刺激实验未出现全身及局部不良反应,组织学观察与阴性对照无显著性差异;皮下植入实验结果显示1周时可见局部组织轻微红肿,组织切片可见少量炎细胞,4周、12周炎症反应随着时间的延长逐渐减轻,试件周围均形成了包膜,随着植入时间的延长包膜逐渐变薄、透明度增高,新生毛细血管的数量增加。各周期中微弧氧化后的锆基非晶合金组皆比实验组的炎细胞数目少,毛细血管数量多,包膜薄,透明度高。说明微弧氧化后的锆基非晶合金组织相容性好,具有良好的临床应用前景。结论作为新型口腔医学植入材料,锆基非晶合金性能优异,但是其生物惰性阻碍了这种材料的应用。微弧氧化处理有效的降低了锆基非晶合金的生物惰性,在保持了锆基非晶合金的优异性能的同时,获得了良好的组织相容性。

Objective Oral mucosa irritation and subcutaneous implantation tests were launched to study the histocompatibility of mi- cro-arc oxidized Zr-Cu-A1-Ag bulk metallic glass （BMG）. Methods Referring to the standard of ISO 10993-6： 1994, micro-arc oxidized BMG and titanium alloy samples with dimension of φ5 mm×0.5 mm for oral mucosa irritation and φ1.5 mm×5 mm for subcutane- ous implantation were prepared, respectively. Results The resuh of oral mucous membrane irritation test showed that all samples did not have local or systemic adverse reactions and the histopathological findings were also normal; the subcutaneous implant test displayed that the Zr-Cu-A1-Ag alloy had no apparent toxicity. After specimens were implanted for one week, local tissue was found to be slightly red and swollen, and the biopsies showed a few of inflammatory cells; 4 and 12 weeks later, it was showed that the inflammatory re- sponse gradually reduced as time prolonged, with the formation process of looser fiber coat. The micro-arc oxidized Zr-base BMG was coated by fiber which was looser than the experimental group. Conclusion As a new type of dental implant materials, the performance of Zr-base BMG is excellent, but its biological inert hinders the application of this material, micro-arc oxidation treatment effectively re- duces the biological inert of Zr-base BMG, maintains the excellent properties of Zr-base BMG, and can give good histocompatibility to Zr-based BMG at the same time.