芪苈强心胶囊对心衰大鼠MMP-2、MMP-9表达的影响

The Effects of Qiliqiangxin Capsules on the Protein Expression of MMP-2,MMP-9 in Rat Heart Failure Model

目的:观察基质金属蛋白酶-2、-9(MMP-2、MMP-9)在心力衰竭大鼠模型中的变化,并采用芪苈强心胶囊进行治疗干预,以确定芪苈强心胶囊对MMPs的影响及其在治疗心力衰竭过程中可能的作用机制。方法:30只雄性SD大鼠随机分为三组:对照组、心力衰竭组(模型组)、心力衰竭+芪苈强心胶囊组(治疗组)。进行以下研究:(1)心脏超声功能改变;(2)大鼠血流动力学变化;(3)组织形态学变化;(4)免疫组化检测心肌组织MMP-2、MMP-9的蛋白表达。结果:模型组大鼠MMP-2、MMP-9蛋白表达的灰度值(119.22±9.13)、(121.31±5.36)显著低于对照组(P<0.01)。治疗组基质中MMP-2、MMP-9的表达灰度值(159.53±5.37)、(155.62±4.57)较模型组显著增高(P<0.01)。结论:芪苈强心胶囊能改善心衰大鼠的心功能及血流动力学指标,这一效应可能与其降低心肌组织中MMP-2、MMP-9的高表达、抑制心肌重塑有关。

Objective： To investigate the changes of matrix metalloproteinases （MMP-2, MMP-9） in rat heart failure model, and the relationships between MMPs and heart failure and the effects of Qiliqiangxin Capsules on it in a rat model. Methods：Thirty male SD rats were randomly divided into three groups： control group, heart failure （model） group and heart failure ＋ Qiliqiangxin Capsules（treated） group. Research： （1）The change of cardiac function evaluated by echocardiography; （2） the change of blood flow dynamics; （3） the change of tissue morphology; （4） the protein ex- pression of MMP-2, MMP-9 were examined by immunohistochemical analysis. Results： The average of the grave scale of protein expression of the MMP-2（119.22±9.13）,MMP-9（121.31±5.36）in model group are lower than these of the control group obviously （P〈0.01） ;after Qiliqiangxin Capsules treatment, the average of the grave scale of protein ex pression of MMP-2 （159. 53±5. 37） ,MMP-9（155. 62±4. 57）are higher than these of the model group prominently （P 〈0. 01）. Conclusion： MMP-2, MMP-9 are involved in the cardiac extracellular matrix remodeling and heart failure. Qil- iqiangxin Capsules can improve heart failure rat cardiac function and hemodynamics, the effect may be related to reduc- ing the high expression of MMP 2 and MMP-9 in myocardial tissue inhibiting myocardial remodeling.