小麦条锈菌滇黔群体遗传结构分析

Population Genetic Structures of Puccinia striiformis f. sp.tritici in Yunnan and Guizhou Province

为探明小麦条锈菌滇黔群体菌源关系,利用扩增片段长度多态性标记对云南、贵州具有不同AFLP基因型的71个小麦条锈菌分离物(云南39个,贵州32个)进行了遗传结构分析。结果表明:小麦条锈菌云南和贵州群体共享AFLP基因型达32.3%;AMOVA分析显示:小麦条锈菌云南和贵州群体间的遗传变异仅占总变异的7%;最大简约树枝长评估揭示小麦条锈菌滇黔群体自由重组的可能性较低。因此,小麦条锈菌滇黔群体间重组几率较低而菌源交流较顺畅。

In order to infer the major migration direction of Puccinia striiformis f. sp. tritici （Pst） between Yunnan and Guizhou populations, amplified fragment length polymorphism （AFLP） was used to analyze population genetic diversity of 71 Pst isolates showing different AFLP genotypes. Among these Pst isolates, 39 isolates were collected from Yunnan Province and 32 from Guizhou Province. There were 32.3% isolates shared AFLP genotypes between Yunnan and Guizhou Pst populations. Only 7% genetic differentiation was observed between the two populations by AMOVA. The most parsimonious tree length test led to rejection of the null hypothesis of free recombination between Yunnan and Guizhou populations. The results indicated that low recombination events and strong gene flow existed between Yunnan and Guizhou populations.