两株喜树内生真菌挥发性代谢物的GC-MS分析

GC-MS Detection of Volatile Metabolites of Two Endophytic Fungi from Camptotheca acuminate

用氯仿∶甲醇(4∶1,体积比)萃取喜树内生真菌X65和X20的发酵液,并用GC-MS联用分析其非极性和极性馏分。结果表明:从上述菌株发酵物中共鉴定出88种挥发性化学成分;其中,非极性馏分60种,主要是烷烃(25种)及霍烷类(15种),其相同组分33种;极性馏分28种,饱和脂肪酸15种,不饱和脂肪酸11种,共有组分15种。菌株X65的极性馏分以饱和脂肪酸类化合物为主,而菌株X20是以不饱和脂肪酸化合物为主;X65鉴定烷烃25种,霍烷类15种,烯烃2种,环烷烃1种,饱和脂肪酸16种,不饱和脂肪酸9种。X20鉴定烷烃24种,霍烷类14种,烯烃2种,甾烷类4种,环烷烃14种,饱和脂肪酸12种,不饱和脂肪酸5种,仲胺1种。

The broths of endophyte fungi （X65 and X20） from Camptotheca acuminata were extracted with chloroform-methanol （4：1, volume ratio）. Non-polarity and polarity volatile compounds were detected by GC-MS. A total of 88 volatile compounds were identified, 33 of which were detected in the two strains. The dominant components in non-polarity compounds were n-al- kanes （25） , followed by alkane Hall （ 15 ） ; 28 polarity compounds were identified, including 15 saturated fatty acids and 11 unsaturated fatty, 15 of which were detected in the two strains. The main polarity compounds of strain X65 and X20 were saturated fatty acids and unsaturated fatty acids, respectively. For the strain X65, 25 alkanes, 2 olefins, 1 cycloalkane, 16 saturated fatty acids, and 9 unsaturated fatty acids were indentified. For the strain X20, 24 alkanes, 14 huo alkanes, 2 olefines, 4 steroid alkanes, 14 naphthenes, 12 saturated fatty acids, 5 unsaturated acids and 1 second amine were indentified.