金铁锁带腋叶嫩茎的组织培养研究

The Tissue Culture Research with Burgeon of Psammosilene tunicoides

以MS为基本培养基,附加不同浓度的植物生长调节剂,将新萌发的带叶腋金铁锁茎段接种于其中,筛选金铁锁最佳组培快速繁殖方案。结果表明:金铁锁外植体消毒最佳方法为75%乙醇消毒0.5 min+0.1%Hg Cl2消毒7 min,污染率可降至35%且成活率可高达73%;金铁锁分化培养最优组合为MS+TDZ 0.01 mg/L+NAA0.5 mg/L,诱导率可达90%;丛生芽增殖培养基以MS+6-BA 0.5 mg/L+NAA 0.5 mg/L最佳,最大增殖苗倍数为22.3个。最适合金铁锁生根的培养为1/2 MS+NAA 0.15 mg/L+IBA 0.1 mg/L,生根率可达95%,且根系生长状况良好。试验结果可为金铁锁快速育苗、种质资源保存、遗传改良提供理论及实践指导。

With MS as the basic culture medium, appended plant growth regulator with different concentrations, the new germination and axillary Psammosilene tunicoides stem section were inoculated in the culture medium, to select the best rapid propagation project of the tissue culture of P. tunicoides. The results showed that： the optimal disinfection combination of P. tunicoides explants was 75qc ethanol continuous 0. 5 rain and 0. 1% HgC12 for 7 rain, pollution rate reduced to 35% and survival rate can be as high as 73%. The optimal combination of differentiation culture of P. tunicoides was MS ＋ 0.01 mg/L TDZ ＋ 0.5mg/L NAA, induction rate could reach 90%. The better proliferation medium of multiple bud clumps was MS ＋ 0.05 mg/L 6-BA ＋ 0.5 mg/L NAA, the biggest proliferation of seedlings reached 22.3 times. The most suitable rooting culture was 1/2 MS ＋ 0. 15 mg/L NAA ＋ 0. 1 mg/L IBA, rooting rate reached 95% and the root growth in good condition. The results provided theoretical and practical guidance for P. tunicoides, including rapid expansion of cultivation, conservation of germptasm resources and genetic improvement.