大球盖菇多糖碱法提取条件优化研究

Optimized Extraction Conditions of Stropharia rugosoannulata Polysaccharides by Alkaline Method

为了探讨大球盖菇多糖碱法提取最佳工艺条件,为大球盖菇多糖的综合开发利用提供参考依据。采用100目菇粉碱液浸提方法,进行碱液浓度（p H）、液料比（m L/g）、温度（℃）和提取时间（min）的单因素试验和四因素三水平[L_9（3^4）]的正交试验,用硫酸苯酚方法测定提取的多糖并计算得率。结果表明：4种因素对大球盖菇多糖提取的影响程度强弱顺序为p H影响最大,时间次之,温度再次之,液料比影响最小。p H 9,提取时间为60 min,温度为80℃,液料比为60 m L/g的组合是大球盖菇多糖提取的最佳条件,大球盖菇多糖的提取得率最高达8.12%。碱法提取是大球盖菇多糖较好的提取方法。该提取工艺条件简单、稳定、可行。

The aim is to explore the optimum extraction conditions of Stropharia rugosoannulata polysaccharide by alkaline method and provide references for S.rugosoannulata polysaccharide utilization. The100 orders powder of S.rugosoannulata was prepared for polysaccharide extraction. The orthogonal test of thesingle factor or four factors including alkaline concentration（p H）, solid to liquid ratio（m L/g）, temperature（℃）and extraction time（min） on three levels was used to determine the optimum extraction method. The yield ofpolysaccharide was calculated by phenol- sulfuric method. The results showed that the extraction yield ofpolysaccharide was affected in order by p H, time, temperature and solid to liquid ratio. The best condition toextract polysaccharide was： p H 9, 60 min of extraction time, 80℃, and 60 m L/g of solid to liquid ratio. Theextraction yield of polysaccharides of S. rugosoannulata reached 8.12%. This means alkaline method ispreferable to extract S. rugosoannulata polysaccharide. At the same time, such extraction process is simple,stable and feasible in S. rugosoannulata polysaccharide extraction.