猕猴桃糖蛋白与芦丁的相互作用研究

Research on the interaction between kiwifruit glycoprotein and rutin

使用荧光光谱法、紫外–可见吸收光谱法、傅里叶红外光谱法和颗粒分析研究猕猴桃糖蛋白(Gp1)与芦丁的相互作用。在不同的两个温度下(300、310 K),由Gp1与芦丁之间的相互作用,探讨二者之间发生作用的荧光猝灭机理以及芦丁对Gp1构象的影响。试验结果表明:芦丁使Gp1发生静态猝灭,二者的相互作用有一个结合位点,芦丁使Gp1主链结构的螺旋结构更为紧密,Gp1二级结构中,α–螺旋、β–转角、β–折叠以及无规则卷曲结构发生不同程度的变化。

The interaction between kiwifruit glycoprotein Gpl and rutin were investigated by fluorescence spectroscopy, ultraviolet visible absorption spectroscopy, Fourier infrared spectroscopy method and particle size analysis. At two different temperatures （300,310 K ）, the fluorescence quenching mechanism and conformation of Gpl were discussed. The results showed that the Gpl was static quenched by rutin. The interaction had one binding site. Rutin made the spiral structure of Gp 1 backbone become more compact. The secondary structure of Gpl, including β-sheet, random coil, α -helix and β-turns had varying degrees of change due to the interaction.